Schmidl, Christian and Hansmann, Leo and Lassmann, Timo and Balwierz, Piotr J. and Kawaji, Hideya and Itoh, Masayoshi and Kawai, Jun and Nagao-Sato, Sayaka and Suzuki, Harukazu and Andreesen, Reinhard and Hayashizaki, Yoshihide and Forrest, Alistair R. R. and Carninci, Piero and Hoffmann, Petra and Edinger, Matthias and Rehli, Michael (2014) The enhancer and promoter landscape of human regulatory and conventional T-cell subpopulations. BLOOD, 123 (17). E68-E78. ISSN 0006-4971, 1528-0020
Full text not available from this repository. (Request a copy)Abstract
CD4(+)CD25(+)FOXP3(+) human regulatory T cells (Tregs) are essential for self-tolerance and immune homeostasis. Here, we describe the promoterome of CD4(+)CD25(high)CD45RA(+) naive and CD4(+)CD25(high)CD45RA(-) memory Tregs and their CD25(-) conventional T-cell (Tconv) counterparts both before and after in vitro expansion by cap analysis of gene expression (CAGE) adapted to single-molecule sequencing (HeliScopeCAGE). We performed comprehensive comparative digital gene expression analyses and revealed novel transcription start sites, of which several were validated as alternative promoters of known genes. For all in vitro expanded subsets, we additionally generated global maps of poised and active enhancer elements marked by histone H3 lysine 4 monomethylation and histone H3 lysine 27 acetylation, describe their cell type-specific motif signatures, and evaluate the role of candidate transcription factors STAT5, FOXP3, RUNX1, and ETS1 in both Treg- and Tconv-specific enhancer architectures. Network analyses of gene expression data revealed additional candidate transcription factors contributing to cell type specificity and a transcription factor network in Tregs that is dominated by FOXP3 interaction partners and targets. In summary, we provide a comprehensive and easily accessible resource of gene expression and gene regulation in human Treg and Tconv subpopulations.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | BONE-MARROW-TRANSPLANTATION; VERSUS-HOST-DISEASE; IN-VITRO EXPANSION; FOXP3 EXPRESSION; SUPPRESSIVE FUNCTION; GENE-EXPRESSION; EX-VIVO; TRANSCRIPTION; RESPONSES; DIFFERENTIATION; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie) Medicine > Regensburger Centrum für Interventionelle Immunologie (RCI) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 13 Nov 2019 15:16 |
| Last Modified: | 13 Nov 2019 15:16 |
| URI: | https://pred.uni-regensburg.de/id/eprint/10293 |
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