Matysik, S. and Schmitz, G. and Bauer, S. and Kiermaier, J. and Matysik, F. -M. (2014) Potential of gas chromatography-atmospheric pressure chemical ionization-time-of-flight mass spectrometry for the determination of sterols in human plasma. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 446 (3). pp. 751-755. ISSN 0006-291X, 1090-2104
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The application of Gas Chromatography (GC)-Atmospheric Pressure Chemical Ionization (APCI)-Time-of-Flight Mass Spectrometry (TOF-MS) is presented for sterol analysis in human plasma. A commercial APCI interface was modified to ensure a well-defined humidity which is essential for controlled ionization. In the first step, optimization regarding flow rates of auxiliary gases was performed by using a mixture of model analytes. Secondly, the qualitative and quantitative analysis of sterols including oxysterols, cholesterol precursors, and plant sterols as trimethylsilyl-derivatives was successfully carried out. The characteristics of APCI together with the very good mass accuracy of TOF-MS data enable the reliable identification of relevant sterols in complex matrices. Linear calibration lines and plausible results for healthy volunteers and patients could be obtained whereas all mass signals were extracted with an extraction. width of 20 ppm from the full mass data set. One advantage of high mass accuracy can be seen in the fact that from one recorded run any search for m/z can be performed. (C) 2014 Elsevier Inc. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CHOLESTEROL PRECURSORS; OXIDATION-PRODUCTS; HUMAN SERUM; ION-SOURCE; OXYSTEROLS; DISEASE; MS; PHYTOSTEROLS; METABOLISM; MS/MS; Oxysterols; APCI; Time-of-flight mass spectrometry |
| Subjects: | 500 Science > 540 Chemistry & allied sciences 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Klinische Chemie und Laboratoriumsmedizin Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Instrumentelle Analytik (Prof. Frank-Michael Matysik) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Nov 2019 10:00 |
| Last Modified: | 14 Nov 2019 10:00 |
| URI: | https://pred.uni-regensburg.de/id/eprint/10314 |
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