Endlich, Nicole and Simon, Ole and Goepferich, Achim and Wegner, Henny and Moeller, Marcus J. and Rumpel, Elisabeth and Kotb, Ahmed M. and Endlich, Karlhans (2014) Two-Photon Microscopy Reveals Stationary Podocytes in Living Zebrafish Larvae. JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY, 25 (4). pp. 681-686. ISSN 1046-6673, 1533-3450
Full text not available from this repository. (Request a copy)Abstract
Podocytes are an essential component of the glomerular filtration barrier and cover the outer aspect of glomerular capillaries. They form a complex actin-based cytoskeleton in vivo and show prominent motility in vitro, but whether podocytes are stationary or mobile in vivo is debated. To address this question, the pronephros of translucent zebrafish larvae (casper) expressing enhanced green fluorescent protein (eGFP) specifically in podocytes (wt1a:eGFP larvae) was observed by intravital two-photon microscopy over extended periods of time. Podocyte cell bodies and the interdigitating branching pattern of major processes could be resolved with a resolution of approximately 1 mu m in the xy-plane. Time-lapse imaging of zebrafish larvae at 5-7 days after fertilization demonstrated that podocytes neither migrated nor changed the branching pattern of their major processes over a time period of up to 23 hours. In summary, we show by extended intravital two-photon microscopy that podocytes are stationary cells in the intact glomerulus of a translucent zebrafish with fluorescently-labeled podocytes.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CELL MOTILITY; ACTIN; MIGRATION; |
| Subjects: | 600 Technology > 615 Pharmacy |
| Divisions: | Chemistry and Pharmacy > Institute of Pharmacy > Pharmaceutical Technology (Prof. Göpferich) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 Nov 2019 09:55 |
| Last Modified: | 15 Nov 2019 09:55 |
| URI: | https://pred.uni-regensburg.de/id/eprint/10392 |
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