Weissenborn, Christine and Ignatov, Tanja and Poehlmann, Angela and Wege, Anja K. and Costa, Serban D. and Zenclussen, Ana Claudia and Ignatov, Atanas (2014) GPER functions as a tumor suppressor in MCF-7 and SK-BR-3 breast cancer cells. JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY, 140 (4). pp. 663-671. ISSN 0171-5216, 1432-1335
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Purpose The orphan, membrane-bound estrogen receptor (GPER) is expressed at high levels in a large fraction of breast cancer patients, and its expression is favorable for patients' survival. We investigated the role of GPER as a potential tumor suppressor in MCF-7 and SK-BR-3 breast cancer cells. Methods The effect of GPER agonist G-1 in cell culture was used to determine whether GPER inhibit cell growth. The methylation status of GPER promoter was investigated by methylation-specific PCR. Results GPER-specific agonist G-1 inhibited breast cancer cell proliferation in concentration-dependent manner via induction of the cell cycle arrest in M-phase, enhanced phosphorylation of histone 3 and cell apoptosis. Analysis of the methylation status of the GPER promoter in MCF-7 and SK-BR-3 cells revealed that GPER expression is regulated by epigenetic mechanisms and GPER expression is inactivated by promoter methylation. Overall, our results are consistent with our recent findings in triple-negative breast cancer cells, and the cell surface expression of GPER makes it an excellent potential therapeutic target for non-triple-negative breast cancer.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PROTEIN-COUPLED RECEPTOR-30; ESTROGEN-RECEPTOR; UP-REGULATION; OVARIAN-CANCER; AGONIST G-1; GENE-EXPRESSION; GPR30; PROLIFERATION; GROWTH; 17-BETA-ESTRADIOL; GPER; GPR30; Breast cancer; Tumor suppressor |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Frauenheilkunde und Geburtshilfe (Schwerpunkt Frauenheilkunde) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 Nov 2019 11:43 |
| Last Modified: | 15 Nov 2019 11:43 |
| URI: | https://pred.uni-regensburg.de/id/eprint/10418 |
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