Wang, Tao and Ge, Yingbin and Xiao, Min and Lopez-Coral, Alfonso and Li, Ling and Roesch, Alexander and Huang, Catherine and Alexander, Peter and Vogt, Thomas and Xu, Xiaowei and Hwang, Wei-Ting and Lieu, Melissa and Belser, Eric and Liu, Rui and Somasundaram, Rajasekharan and Herlyn, Meenhard and Kaufman, Russel E. (2014) SECTM1 Produced by Tumor Cells Attracts Human Monocytes via CD7-Mediated Activation of the PI3K Pathway. JOURNAL OF INVESTIGATIVE DERMATOLOGY, 134 (4). pp. 1108-1118. ISSN 0022-202X, 1523-1747
Full text not available from this repository.Abstract
Tumor-associated macrophages (TAMs) have essential roles in tumor progression and metastasis. Tumor cells recruit myeloid progenitors and monocytes to the tumor site, where they differentiate into TAMs; however, this process is not well studied in humans. Here we show that human CD7, a T-cell and NK cell receptor, is highly expressed by monocytes and macrophages. Expression of CD7 decreases in M-CSF-differentiated macrophages and in melanoma-conditioned medium-induced macrophages (MCMI/Mf) in comparison to monocytes. A ligand for CD7, SECTM1 (secreted and transmembrane protein 1), is highly expressed in many tumors, including melanoma cells. We show that SECTM1 binds to CD7 and significantly increases monocyte migration by activation of the PI3K (phosphatidylinositol 30-kinase) pathway. In human melanoma tissues, tumor-infiltrating macrophages expressing CD7 are present. These melanomas, with CD7-positive inflammatory cell infiltrations, frequently highly express SECTM1, including an N-terminal, soluble form, which can be detected in the sera of metastatic melanoma patients but not in normal sera. Taken together, our data demonstrate that CD7 is present on monocytes and tumor macrophages and that its ligand, SECTM1, is frequently expressed in corresponding melanoma tissues, possibly acting as a chemoattractant for monocytes to modulate the melanoma microenvironment.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MALIGNANT-MELANOMA; MACROPHAGE INFILTRATION; K12 SECTM1; CD7; MOLECULE; CANCER; GAMMA; GENE; DIFFERENTIATION; IDENTIFICATION; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Dermatologie und Venerologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 15 Nov 2019 11:57 |
| Last Modified: | 15 Nov 2019 11:57 |
| URI: | https://pred.uni-regensburg.de/id/eprint/10421 |
Actions (login required)
 |
View Item |
