Haussner, Christina and Damm, Dominik and Nirschl, Sandra and Rohrhofer, Anette and Schmidt, Barbara and Eichler, Jutta (2017) Peptide Paratope Mimics of the Broadly Neutralizing HIV-1 Antibody b12. CHEMBIOCHEM, 18 (7). pp. 647-653. ISSN 1439-4227, 1439-7633
Full text not available from this repository. (Request a copy)Abstract
The broadly neutralizing HIV-1 antibody b12 recognizes the CD4 binding site of the HIV-1 envelope glycoprotein gp120 and efficiently neutralizes HIV-1 infections in vitro and in vivo. Based on the 3D structure of a b12-gp120 complex, we have designed an assembled peptide (b12-M) that presents the parts of the three heavy-chain complementarity-determining regions (CDRs) of b12, which contain the contact sites of the antibody for gp120. This b12-mimetic peptide, as well as a truncated peptide presenting only two of the three heavy-chain CDRs of b12, were shown to recognize gp120 in a similar manner to b12, as well as to inhibit HIV-1 infection, demonstrating functional mimicry of b12 by the paratope mimetic peptides.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | IMMUNODEFICIENCY-VIRUS; MONOCLONAL-ANTIBODY; IN-VITRO; ENVELOPE GLYCOPROTEINS; DESIGN; GP120; IMMUNOGENS; INFECTION; RESPONSES; BINDING; antibody mimics; gp120; HIV-1; immunochemistry; MAb b12; peptides |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Dec 2018 13:11 |
| Last Modified: | 11 Feb 2019 14:11 |
| URI: | https://pred.uni-regensburg.de/id/eprint/1049 |
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