Spoerner, Michael and Karl, Matthias and Lopes, Pedro and Hoering, Marcus and Loeffel, Karoline and Nuehs, Andrea and Adelsberger, Joseph and Kremer, Werner and Kalbitzer, Hans Robert (2017) High pressure P-31 NMR spectroscopy on guanine nucleotides. JOURNAL OF BIOMOLECULAR NMR, 67 (1). pp. 1-13. ISSN 0925-2738, 1573-5001
Full text not available from this repository. (Request a copy)Abstract
The P-31 NMR pressure response of guanine nucleotides bound to proteins has been studied in the past for characterizing the pressure perturbation of conformational equilibria. The pressure response of the P-31 NMR chemical shifts of the phosphate groups of GMP, GDP, and GTP as well as the commonly used GTP analogs GppNHp, GppCH(2)p and GTP gamma S was measured in the absence and presence of Mg2+-ions within a pressure range up to 200 MPa. The pressure dependence of chemical shifts is clearly non-linear. For all nucleotides a negative first order pressure coefficient B (1) was determined indicating an upfield shift of the resonances with pressure. With exception of the alpha-phosphate group of Mg2+center dot GMP and Mg2+center dot GppNHp the second order pressure coefficients are positive. To describe the data of Mg2+center dot GppCH(2)p and GTP gamma S a Taylor expansion of 3rd order is required. For distinguishing pH effects from pressure effects a complete pH titration set is presented for GMP, as well as GDP and GTP in absence and presence of Mg2+ ions using indirect referencing to DSS under identical experimental conditions. By a comparison between high pressure P-31 NMR data on free Mg2+-GDP and Mg2+-GDP in complex with the proto-oncogene Ras we demonstrate that pressure induced changes in chemical shift are clearly different between both forms.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | NUCLEAR-MAGNETIC-RESONANCE; CONFORMATIONAL STATES; RAS PROTEIN; EFFECTOR INTERACTION; CHEMICAL-SHIFTS; BINDING; GTP; COMPLEXES; DYNAMICS; PERTURBATION; P-31 NMR; High pressure NMR spectroscopy; Guanine nucleotide; GMP; GDP; GTP; GTP analogs |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie > Prof. Dr. Dr. Hans Robert Kalbitzer |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Dec 2018 12:58 |
| Last Modified: | 13 Feb 2019 13:20 |
| URI: | https://pred.uni-regensburg.de/id/eprint/105 |
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