Grosse, Jirka and Warnke, Elisabeth and Wehland, Markus and Pietsch, Jessica and Pohl, Fabian and Wise, Petra and Magnusson, Nils E. and Eilles, Christoph and Grimm, Daniela (2014) Mechanisms of apoptosis in irradiated and sunitinib-treated follicular thyroid cancer cells. APOPTOSIS, 19 (3). pp. 480-490. ISSN 1360-8185, 1573-675X
Full text not available from this repository. (Request a copy)Abstract
The multikinase inhibitor sunitinib (S) seems to have promising potential in the treatment of thyroid cancer. We focused on the impact of S and/or irradiation (R) on mechanisms of apoptosis in follicular thyroid cancer cells. The effects of R, S and their combination were evaluated 2 and 4 days after treatment, using the human thyroid cancer cell line CGTH W-1. The transcription of genes involved in the regulation of apoptosis was investigated using quantitative real-time PCR. Western blot analyses of caspases and survivin were also performed. S elevated BAX (day 4), CASP9, CASP3, BIRC5 (day 4) and PRKACA (day 4) gene expression, whereas the mRNAs of BCL2, CASP8, PRKCA, ERK1, and ERK2 were not significantly changed. S, R and R+S clearly induced caspase-9 protein and elevated caspase-3 activity. Survivin was down-regulated at day 4 in control cells and the expression was blunted by S treatment. R+S induced survivin expression at day 2 followed by a reduction at day 4 of treatment. Sunitinib and the combined application with radiation induced apoptosis in follicular thyroid cancer cells via the intrinsic pathway of apoptosis. In addition, sunitinib might induce apoptosis via decreased expression of the anti-apoptotic protein survivin. These findings suggest the potential use of sunitinib for the treatment of poorly differentiated follicular thyroid carcinomas.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | BREAST-CANCER; THERAPEUTIC TARGET; ENDOTHELIAL-CELLS; PARABOLIC FLIGHT; KINASE INHIBITOR; BCL-2 FAMILY; SURVIVIN; CARCINOMA; DEATH; GENE; Thyroid cancer; Sunitinib; Irradiation; Apoptosis; Survivin; Caspases |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Strahlentherapie Medicine > Abteilung für Nuklearmedizin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 26 Nov 2019 13:06 |
| Last Modified: | 26 Nov 2019 13:06 |
| URI: | https://pred.uni-regensburg.de/id/eprint/10605 |
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