Breil, Cassandra and Vian, Maryline Abert and Zemb, Thomas and Kunz, Werner and Chemat, Farid (2017) Bligh and Dyer and Folch Methods for Solid-Liquid-Liquid Extraction of Lipids from Microorganisms. Comprehension of Solvatation Mechanisms and towards Substitution with Alternative Solvents. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 18 (4): 708. ISSN 1422-0067,
Full text not available from this repository. (Request a copy)Abstract
Bligh and Dyer (B & D) or Folch procedures for the extraction and separation of lipids from microorganisms and biological tissues using chloroform/methanol/water have been used tens of thousands of times and are "gold standards" for the analysis of extracted lipids. Based on the Conductor-like Screening MOdel for realistic Solvatation (COSMO-RS), we select ethanol and ethyl acetate as being potentially suitable for the substitution of methanol and chloroform. We confirm this by performing solid-liquid extraction of yeast (Yarrowia lipolytica IFP29) and subsequent liquid-liquid partition-the two steps of routine extraction. For this purpose, we consider similar points in the ternary phase diagrams of water/methanol/chloroform and water/ethanol/ethyl acetate, both in the monophasic mixtures and in the liquid-liquid miscibility gap. Based on high performance thin-layer chromatography (HPTLC) to obtain the distribution of lipids classes, and gas chromatography coupled with a flame ionisation detector (GC/FID) to obtain fatty acid profiles, this greener solvents pair is found to be almost as effective as the classic methanol-chloroform couple in terms of efficiency and selectivity of lipids and non-lipid material. Moreover, using these bio-sourced solvents as an alternative system is shown to be as effective as the classical system in terms of the yield of lipids extracted from microorganism tissues, independently of their apparent hydrophilicity.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | BIO-BASED SOLVENTS; CELL-WALL; SACCHAROMYCES-CEREVISIAE; RAPID METHOD; COSMO-RS; CELLULOSIC BIOETHANOL; YEAST; MICROWAVE; PURIFICATION; ULTRASOUND; Bligh and Dyer; Folch; bio-sourced solvent; yeast; lipids |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Physikalische und Theoretische Chemie > Chair of Chemistry VI - Physical Chemistry (Solution Chemistry) > Prof. Dr. Werner Kunz |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Dec 2018 13:11 |
| Last Modified: | 28 Feb 2019 10:46 |
| URI: | https://pred.uni-regensburg.de/id/eprint/1071 |
Actions (login required)
 |
View Item |
