Delic, Sabit and Lottmann, Nadine and Stelzl, Anja and Liesenberg, Franziska and Wolter, Marietta and Goetze, Silke and Zapatka, Marc and Shiio, Yuzuru and Sabel, Michael C. and Felsberg, Joerg and Reifenberger, Guido and Riemenschneider, Markus J. (2014) MiR-328 promotes glioma cell invasion via SFRP1-dependent Wnt-signaling activation. NEURO-ONCOLOGY, 16 (2). pp. 179-190. ISSN 1522-8517, 1523-5866
Full text not available from this repository. (Request a copy)Abstract
Background. Diffusely infiltrative growth of human astrocytic gliomas is one of the major obstacles to successful tumor therapy. Thorough insights into the molecules and pathways signaling glioma cell invasion thus appear of major relevance for the development of targeted and individualized therapies. By miRNA expression profiling of microdissected human tumor biopsy specimens we identified miR-328 as one of the main miRNAs upregulated in invading glioma cells in vivo and further investigated its role in glioma pathogenesis. Methods. We employed miRNA mimics and inhibitors to functionally characterize miR-328, 3' untranslated region luciferase assays, and T-cell factor/lymphoid enhancer factor reporter assays to pinpoint miR-328 targets and signaling pathways, and analyzed miR-328 expression in a large panel of gliomas. Results. First, we corroborated the invasion-promoting role of miR-328 in A172 and TP365MG glioma cells. Secreted Frizzled-related protein1(SFRP1), aninhibitor of Wnt signaling, was then pinpointed as a direct miR-328 target. SFRP1 expression is of prognostic relevance in gliomas with reduced expression, being associated with significantly lower overall patient survival in both the Repository of Molecular Brain Neoplasia Data (REMBRANDT) and The Cancer Genome Atlas. Of note, miR-328 regulated both SFRP1 protein expression levels and Wnt signaling pathway activity. Finally, in human glioma tissues miR-328 appeared to account for the downregulation of SFRP1 preferentially in lower-grade astrocytic gliomas and was inversely related to SFRP1 promoter hypermethylation. Conclusion. Taken together, we report on a novel molecular miR-328-dependent mechanism that via SFRP1 inhibition and Wnt activation contributes to the infiltrative glioma phenotype at already early stages of glioma progression, with unfavorable prognostic implications for the final outcome of the disease.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | DOWN-REGULATION; MOLECULAR TARGETS; ANTAGONIST SFRP1; CERVICAL-CANCER; EXPRESSION; HYPERMETHYLATION; PATHWAY; GROWTH; GENES; PROLIFERATION; astrocytoma; brain tumor; epigenetic; glioblastoma; miRNA |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Abteilung für Neuropathologie Medicine > Zentren des Universitätsklinikums Regensburg > Zentrum für Hirntumore (ZHT) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 28 Nov 2019 09:30 |
| Last Modified: | 28 Nov 2019 09:30 |
| URI: | https://pred.uni-regensburg.de/id/eprint/10753 |
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