Meador, Travis B. and Gagen, Emma J. and Loscar, Michael E. and Goldhammer, Tobias and Yoshinaga, Marcos Y. and Wendt, Jenny and Thomm, Michael and Hinrichs, Kai-Uwe (2014) Thermococcus kodakarensis modulates its polar membrane lipids and elemental composition according to growth stage and phosphate availability. FRONTIERS IN MICROBIOLOGY, 5: 10. ISSN 1664-302X,
Full text not available from this repository. (Request a copy)Abstract
We observed significant changes in the elemental and intact polar lipid (IPL) composition of the archaeon Thermococcus kodakarensis (KOD1) in response to growth stage and phosphorus supply. Reducing the amount of organic supplements and phosphate in growth media resulted in significant decreases in cell size and cellular quotas of carbon (C), nitrogen (N), and phosphorus (P), which coincided with significant increases in cellular IPL quota and IPLs comprising multiple P atoms and hexose moieties. Relatively more cellular P was stored as IPLs in P-limited cells (2-8%) compared to control cells (<0.8%). We also identified a specific IPL biomarker containing a phosphatidyl-N-acetylhexoseamine headgroup that was relatively enriched during rapid cell division. These observations serve as empirical evidence of IPL adaptations in Archaea that will help to interpret the distribution of these biomarkers in natural systems. The reported cell quotas of C, N, and P represent the first such data for a specific archaeon and suggest that thermophiles are C-rich compared to the cell carbon-to-volume relationship reported for planktonic bacteria.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MARINE SUBSURFACE SEDIMENTS; MASS-SPECTROMETRY; METHANOBACTERIUM-THERMOAUTOTROPHICUM; MICROBIAL COMMUNITIES; MESOPELAGIC OCEAN; ARCHAEA; TETRAETHER; TEMPERATURE; BACTERIA; DIETHER; biomarker; phosphorus limitation; cell carbon quota |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 28 Nov 2019 09:57 |
| Last Modified: | 28 Nov 2019 09:57 |
| URI: | https://pred.uni-regensburg.de/id/eprint/10784 |
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