Reisinger, Bernd and Sperl, Josef and Holinski, Alexandra and Schmid, Veronika and Rajendran, Chitra and Carstensen, Linn and Schlee, Sandra and Blanquart, Samuel and Merkl, Rainer and Sterner, Reinhard (2014) Evidence for the Existence of Elaborate Enzyme Complexes in the Paleoarchean Era. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 136 (1). pp. 122-129. ISSN 0002-7863,
Full text not available from this repository. (Request a copy)Abstract
Due to the lack of macromolecular fossils, the enzymatic repertoire of extinct species has remained largely unknown to date. In an attempt to solve this problem, we have characterized a cydase subunit (HisF) of the imidazole glycerol phosphate synthase (ImGP-S), which was reconstructed from the era of the last universal common ancestor of cellular organisms (LUCA). As observed for contemporary HisF proteins, the crystal structure of LUCA-HisF adopts the (beta alpha)(8)-barrel architecture, one of the most ancient folds. Moreover, LUCA-HisF (i) resembles extant HisF proteins with regard to internal 2-fold symmetry, active site residues, and a stabilizing salt bridge cluster, (ii) is thermostable and shows a folding mechanism similar to that of contemporary (beta alpha)(8)-barrel enzymes, (iii) displays high catalytic activity, and (iv) forms a stable and functional complex with the glutaminase subunit (HisH) of an extant ImGP-S. Furthermore, we show that LUCA-HisF binds to a reconstructed LUCA-HisH protein with high affinity. Our findings suggest that the evolution of highly efficient enzymes and enzyme complexes has already been completed in the LUCA era, which means that sophisticated catalytic concepts such as substrate channeling and allosteric communication existed already 3.5 billion years ago.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | GLYCEROL PHOSPHATE SYNTHASE; (BETA-ALPHA)(8)-BARREL ENZYME; TRYPTOPHAN BIOSYNTHESIS; SEQUENCE ALIGNMENT; GLUTAMINE AMIDOTRANSFERASE; STRUCTURAL EVIDENCE; FOLDING MECHANISM; PROTEIN; EVOLUTION; HISTIDINE; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie > Prof. Dr. Reinhard Sterner Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie > Prof. Dr. Rainer Merkl |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 28 Nov 2019 10:22 |
| Last Modified: | 28 Nov 2019 10:22 |
| URI: | https://pred.uni-regensburg.de/id/eprint/10827 |
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