Birner, Christoph and Hierl, Sarah and Dietl, Alexander and Hupf, Julian and Jungbauer, Carsten and Schmid, Peter M. and Ruemmele, Petra and Deutzmann, Rainer and Riegger, Guenter and Luchner, Andreas (2014) Experimental Heart Failure Induces Alterations of the Lung Proteome - Insight into Molecular Mechanisms. CELLULAR PHYSIOLOGY AND BIOCHEMISTRY, 33 (3). pp. 692-704. ISSN 1015-8987, 1421-9778
Full text not available from this repository. (Request a copy)Abstract
Background: Heart failure (CHF) is characterized by dyspnea and pulmonary changes. The underlying molecular adaptations are unclear, but might provide targets for therapeutic interventions. We therefore conceived a study to determine molecular changes of early pulmonary stress failure in a model of tachycardia-induced heart failure. Methods: CHF was induced in rabbits by progessive right ventricular pacing (n=6). Invasive blood pressure measurements and echocardiography were repeatedly performed. Untreated animals served as controls (n=6). Pulmonary tissue specimens were subjected to two dimensional gel electrophoresis, and differentially expressed proteins were identified by mass spectrometry. Selected proteins were validated by Western Blot analysis and localized by immunohistochemical staining. Results: CHF animals were characterized by significantly altered functional, morphological, and hemodynamic parameters. Upon proteomic profiling, a total of 33 proteins was found to be differentially expressed in pulmonary tissue of CHF animals (18 up-regulated, and 15 down-regulated) belonging to 4 functional groups: 1. proteins involved in maintaining cytoarchitectural integrity, 2. plasma proteins indicating impaired alveolar-capillary permeability, 3. proteins with antioxidative properties, and 4. proteins participating in the metabolism of selenium compounds Conclusion: Experimental heart failure profoundly alters the pulmonary proteome. Our results supplement the current knowledge of pulmonary stress failure by specifying its molecular fundament.Copyright (C) 2014 S. Karger AG, Basel
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CAPILLARY MEMBRANE DYSFUNCTION; 2-DIMENSIONAL ELECTROPHORESIS; EZRIN/RADIXIN/MOESIN PROTEINS; DIFFERENTIAL EXPRESSION; STRESS; PERMEABILITY; CELLS; ABNORMALITIES; CYTOSKELETON; INCREASES; Experimental heart failure; Proteomics; Lung |
| Subjects: | 500 Science > 570 Life sciences 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin II Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie I > Prof. Dr. Rainer Deutzmann |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 29 Nov 2019 09:23 |
| Last Modified: | 29 Nov 2019 09:23 |
| URI: | https://pred.uni-regensburg.de/id/eprint/11005 |
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