Tremblay, Maxime and Charton, Romain and Wittner, Manuel and Levasseur, Genevieve and Griesenbeck, Joachim and Conconi, Antonio (2014) UV light-induced DNA lesions cause dissociation of yeast RNA polymerases-I and establishment of a specialized chromatin structure at rRNA genes. NUCLEIC ACIDS RESEARCH, 42 (1). pp. 380-395. ISSN 0305-1048, 1362-4962
Full text not available from this repository. (Request a copy)Abstract
The cytotoxicity of UV light-induced DNA lesions results from their interference with transcription and replication. DNA lesions arrest elongating RNA polymerases, an event that triggers transcription-coupled nucleotide excision repair. Since arrested RNA polymerases reduce the accessibility of repair factors to DNA lesions, they might be displaced. The fate of arrested RNA polymerases-II at DNA lesions has been extensively studied, yielding partially contradictory results. Considerably less is known about RNA polymerases-I that transcribe nucleosomes-depleted rRNA genes at very high rate. To investigate the fate of arrested RNA polymerases-I at DNA lesions, chromatin-immunoprecipitation, electron microscopy, transcription run-on, psoralen-cross-linking and chromatin-endogenous cleavage were employed. We found that RNA polymerases-I density increased at the 5'-end of the gene, likely due to continued transcription initiation followed by elongation and pausing/release at the first DNA lesion. Most RNA polymerases-I dissociated downstream of the first DNA lesion, concomitant with chromatin closing that resulted from deposition of nucleosomes. Although nucleosomes were deposited, the high mobility group-box Hmo1 (component of actively transcribed rRNA genes) remained associated. After repair of DNA lesions, Hmo1 containing chromatin might help to restore transcription elongation and reopening of rRNA genes chromatin.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | NUCLEOTIDE EXCISION-REPAIR; SACCHAROMYCES-CEREVISIAE; CELL-CYCLE; DHFR GENE; TRANSCRIPTION; DAMAGE; REPLICATION; ELONGATION; INITIATION; RDNA; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie III > Dr. Joachim Griesenbeck |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 29 Nov 2019 11:58 |
| Last Modified: | 29 Nov 2019 11:58 |
| URI: | https://pred.uni-regensburg.de/id/eprint/11049 |
Actions (login required)
 |
View Item |
