Antosz, Wojciech and Pfab, Alexander and Ehrnsberger, Hans F. and Holzinger, Philipp and Koellen, Karin and Mortensen, Simon A. and Bruckmann, Astrid and Schubert, Thomas and Laengst, Gernot and Griesenbeck, Joachim and Schubert, Veit and Grasser, Marion and Grasser, Klaus D. (2017) The Composition of the Arabidopsis RNA Polymerase II Transcript Elongation Complex Reveals the Interplay between Elongation and mRNA Processing Factors. PLANT CELL, 29 (4). pp. 854-870. ISSN 1040-4651, 1532-298X
Full text not available from this repository. (Request a copy)Abstract
Transcript elongation factors (TEFs) are a heterogeneous group of proteins that control the efficiency of transcript elongation of subsets of genes by RNA polymerase II (RNAPII) in the chromatin context. Using reciprocal tagging in combination with affinity purification and mass spectrometry, we demonstrate that in Arabidopsis thaliana, the TEFs SPT4/SPT5, SPT6, FACT, PAF1-C, and TFIIS copurified with each other and with elongating RNAPII, while P-TEFb was not among the interactors. Additionally, NAP1 histone chaperones, ATP-dependent chromatin remodeling factors, and some histone-modifying enzymes including Elongator were repeatedly found associated with TEFs. Analysis of double mutant plants defective in different combinations of TEFs revealed genetic interactions between genes encoding subunits of PAF1-C, FACT, and TFIIS, resulting in synergistic/epistatic effects on plant growth/development. Analysis of subnuclear localization, gene expression, and chromatin association did not provide evidence for an involvement of the TEFs in transcription by RNAPI (or RNAPIII). Proteomics analyses also revealed multiple interactions between the transcript elongation complex and factors involved in mRNA splicing and polyadenylation, including an association of PAF1-C with the polyadenylation factor CstF. Therefore, the RNAPII transcript elongation complex represents a platform for interactions among different TEFs, as well as for coordinating ongoing transcription with mRNA processing.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | DIRECTED DNA METHYLATION; LOCUS-C CHROMATIN; PAF1 COMPLEX; P-TEFB; GENE-EXPRESSION; SACCHAROMYCES-CEREVISIAE; AFFINITY PURIFICATION; PLANT-GROWTH; SH2 DOMAIN; PROTEIN; |
| Subjects: | 500 Science > 570 Life sciences 500 Science > 580 Botanical sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Lehrstuhl für Zellbiologie und Pflanzenphysiologie Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie I Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie III Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie III > Dr. Joachim Griesenbeck |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Dec 2018 13:11 |
| Last Modified: | 28 Feb 2019 11:22 |
| URI: | https://pred.uni-regensburg.de/id/eprint/1106 |
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