Gehmert, Sebastian and Wenzel, Carina and Loibl, Markus and Brockhoff, Gero and Huber, Michaela and Krutsch, Werner and Nerlich, Michael and Gosau, Martin and Klein, Silvan and Schreml, Stephan and Prantl, Lukas and Gehmert, Sanga (2014) Adipose Tissue-Derived Stem Cell Secreted IGF-1 Protects Myoblasts from the Negative Effect of Myostatin. BIOMED RESEARCH INTERNATIONAL: 129048. ISSN 2314-6133, 2314-6141
Full text not available from this repository. (Request a copy)Abstract
Myostatin, a TGF-beta family member, is associated with inhibition of muscle growth and differentiation and might interact with the IGF-1 signaling pathway. Since IGF-1 is secreted at a bioactive level by adipose tissue-derived mesenchymal stem cells (ASCs), these cells (ASCs) provide a therapeutic option for Duchenne Muscular Dystrophy (DMD). But the protective effect of stem cell secreted IGF-1 on myoblast under high level of myostatin remains unclear. In the present study murine myoblasts were exposed to myostatin under presence of ASCs conditioned medium and investigated for proliferation and apoptosis. The protective effect of IGF-1 was further examined by using IGF-1 neutralizing and receptor antibodies as well as gene silencing RNAi technology. MyoD expression was detected to identify impact of IGF-1 on myoblasts differentiation when exposed to myostatin. IGF-1 was accountable for 43.6% of the antiapoptotic impact and 48.8% for the proliferative effect of ASCs conditioned medium. Furthermore, IGF-1 restored mRNA and protein MyoD expression of myoblasts under risk. Beside fusion and transdifferentiation the beneficial effect of ASCs is mediated by paracrine secreted cytokines, particularly IGF-1. The present study underlines the potential of ASCs as a therapeutic option for Duchenne muscular dystrophy and other dystrophic muscle diseases.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | DUCHENNE MUSCULAR-DYSTROPHY; INDUCED MUSCLE DAMAGE; SKELETAL-MUSCLE; CONTRACTILE FUNCTION; GENE-EXPRESSION; CYCLIN D1; GROWTH; DIFFERENTIATION; PROLIFERATION; MICE; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Unfallchirurgie Medicine > Lehrstuhl für Dermatologie und Venerologie Medicine > Lehrstuhl für Frauenheilkunde und Geburtshilfe (Schwerpunkt Frauenheilkunde) Medicine > Lehrstuhl für Mund-, Kiefer- und Gesichtschirurgie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 02 Dec 2019 10:07 |
| Last Modified: | 02 Dec 2019 10:07 |
| URI: | https://pred.uni-regensburg.de/id/eprint/11064 |
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