Morsczeck, C. and Reck, A. and Beck, H. C. (2017) The hedgehog-signaling pathway is repressed during the osteogenic differentiation of dental follicle cells. MOLECULAR AND CELLULAR BIOCHEMISTRY, 428 (1-2). pp. 79-86. ISSN 0300-8177, 1573-4919
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Dental follicle stem cells (DFCs) are precursor cells of alveolar osteoblasts, and previous studies have shown that the growth factor bone morphogenetic protein (BMP)2 induces the osteogenic differentiation of DFCs. However, the molecular mechanism down-stream of the induction of the osteogenic differentiation by BMP2 remains elusive. We investigated therefore the phosphoproteome of DFCs after the induction of the osteogenic differentiation with BMP2. In this study, phosphoproteins of the hedgehog "off" state were differentially expressed. Further analyses revealed that BMP2 induced the expression of repressors of the hedgehog-signaling pathway such as Patched 1 (PTCH1), Suppressor of Fused (SUFU), and Parathyroid Hormone-Related Peptide (PTHrP). Previous studies suggested that hedgehog proteins induce the osteogenic differentiation of mesenchymal stem cells via a paracrine pathway. Indian hedgehog (IHH) induced the expression of the osteogenic transcription factor RUNX2. However, a supplementation of the BMP2-based osteogenic differentiation medium with IHH did not induce the expression of RUNX2. Moreover, IHH inhibited slightly the ALP activity and the mineralization of osteogenic-differentiated DFCs. In conclusion, our results suggest that BMP2 inhibits the hedgehog signaling after the induction of the osteogenic differentiation in DFCs.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PRECURSOR CELLS; ALKALINE-PHOSPHATASE; STEM-CELLS; TOOTH ROOT; EXPRESSION; PROTEIN; CHALLENGES; MOUSE; TEETH; BONE; Hedgehog-signaling pathway; Dental follicle cells; Osteogenic differentiation; Periodontium; Bone morphogenetic protein |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Herz-, Thorax- und herznahe Gefäßchirurgie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Dec 2018 13:11 |
| Last Modified: | 19 Feb 2019 06:44 |
| URI: | https://pred.uni-regensburg.de/id/eprint/1126 |
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