Mattarei, Andrea and Enzinger, Monika and Gu, Siyi and Karunakaran, Mohindar Murugesh and Kimmel, Brigitte and Berner, Nicole and Adams, Erin J. and Herrmann, Thomas and Amslinger, Sabine (2017) A Photo-Crosslinkable Biotin Derivative of the Phosphoantigen (E)-4-Hydroxy-3-Methylbut-2-Enyl Diphosphate (HMBPP) Activates V gamma 9V delta 2 T Cells and Binds to the HMBPP Site of BTN3A1. CHEMISTRY-A EUROPEAN JOURNAL, 23 (49). pp. 11945-11954. ISSN 0947-6539, 1521-3765
Full text not available from this repository. (Request a copy)Abstract
V gamma 9V delta 2 T cells play an important role in the cross talk of the innate and adaptive immune system. For their activation by phosphoantigens (PAgs), both cell surface receptors, the eponymous V gamma 9V delta 2 T cell antigen receptors (V gamma 9V delta 2 TCRs) on V gamma 9V delta 2 T cells and butyrophilin 3A1 (BTN3A1) on the phosphoantigen-"presenting" cell, are mandatory. To find yet undetected but further contributing proteins, a biotinylated, photo-crosslinkable benzophenone probe BioBP-HMBPP (2) was synthesized from a known allyl alcohol in nine steps and overall 16% yield. 2 is based on the picomolar PAg (E)-4-hydroxy-3-methylbut-2-enyl diphosphate (HMBPP, 1). Laser irradiation of 2 at 308nm initiated the photo-crosslinking reaction with proteins. When the B30.2 domain of BTN3A1, which contains a positively charged PAg-binding pocket, was exposed to increasing amounts of HMBPP (1), labeling by BioBP-HMBPP (2) was reduced significantly. Because BSA labeling was not impaired, 2 clearly binds to the same site as natural ligand 1. Thus, BioBP-HMBPP (2) is a suitable tool to identify co-ligands or receptors involved in PAg-mediated T cell activation.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | (E)-1-HYDROXY-2-METHYL-BUT-2-ENYL 4-DIPHOSPHATE; PHOSPHORYLATED ANTIGENS; TUMOR-CELLS; PYROPHOSPHATE; STIMULATION; RECOGNITION; PRENYLTRANSFERASES; BIOSYNTHESIS; SYNTHASE; RECEPTOR; B30.2 domain; BTN3A1; HMBPP; phosphoantigen; photoaffinity label |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Organische Chemie > Arbeitskreis Dr. Sabine Amslinger |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Dec 2018 13:15 |
| Last Modified: | 11 Feb 2019 14:25 |
| URI: | https://pred.uni-regensburg.de/id/eprint/1195 |
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