Chen, Junyi and Strieder, Nicholas and Krohn, Nadia G. and Cyprys, Philipp and Sprunck, Stefanie and Engelmann, Julia C. and Dresselhaus, Thomas (2017) Zygotic Genome Activation Occurs Shortly after Fertilization in Maize. PLANT CELL, 29 (9). pp. 2106-2125. ISSN 1040-4651, 1532-298X
Full text not available from this repository. (Request a copy)Abstract
The formation of a zygote via the fusion of an egg and sperm cell and its subsequent asymmetric division herald the start of the plant's life cycle. Zygotic genome activation (ZGA) is thought to occur gradually, with the initial steps of zygote and embryo development being primarily maternally controlled, and subsequent steps being governed by the zygotic genome. Here, using maize (Zea mays) as a model plant system, we determined the timing of zygote development and generated RNA-seq transcriptome profiles of gametes, zygotes, and apical and basal daughter cells. ZGA occurs shortly after fertilization and involves similar to 10% of the genome being activated in a highly dynamic pattern. In particular, genes encoding transcriptional regulators of various families are activated shortly after fertilization. Further analyses suggested that chromatin assembly is strongly modified after fertilization, that the egg cell is primed to activate the translational machinery, and that hormones likely play a minor role in the initial steps of early embryo development in maize. Our findings provide important insights into gamete and zygote activity in plants, and our RNA-seq transcriptome profiles represent a comprehensive, unique RNA-seq data set that can be used by the research community.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | APICAL-BASAL AXIS; DNA-REPLICATION; WIDE ANALYSIS; ZEA-MAYS; EARLY EMBRYOGENESIS; SPERM CELLS; ARABIDOPSIS EMBRYO; FLOWERING PLANTS; PATERNAL GENOMES; MCM PROTEINS; |
| Subjects: | 500 Science > 580 Botanical sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Dec 2018 13:15 |
| Last Modified: | 28 Feb 2019 11:29 |
| URI: | https://pred.uni-regensburg.de/id/eprint/1216 |
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