Dillinger, Andrea E. and Guter, Michaela and Froemel, Franziska and Weber, Gregor R. and Perkumas, Kristin and Stamer, W. Daniel and Ohlmann, Andreas and Fuchshofer, Rudolf and Breunig, Miriam (2018) Intracameral Delivery of Layer-by-Layer Coated siRNA Nanoparticles for Glaucoma Therapy. SMALL, 14 (50): 1803239. ISSN 1613-6810, 1613-6829
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Glaucoma is the second leading cause of blindness worldwide, often associated with elevated intraocular pressure. Connective tissue growth factor (CTGF) is a mediator of pathological effects in the trabecular meshwork (TM) and Schlemm's canal (SC). A novel, causative therapeutic concept which involves the intracameral delivery of small interfering RNA against CTGF is proposed. Layer-by-layer coated nanoparticles of 200-260 nm with a final layer of hyaluronan (HA) are developed. The HA-coating should provide the nanoparticles sufficient mobility in the extracellular matrix and allow for binding to TM and SC cells via CD44. By screening primary TM and SC cells in vitro, in vivo, and ex vivo, the validity of the concept is confirmed. CD44 expression is elevated in glaucomatous versus healthy cells by about two- to sixfold. CD44 is significantly involved in the cellular uptake of HA-coated nanoparticles. Ex vivo organ culture of porcine, murine, and human eyes demonstrates up to threefold higher accumulation of HA compared to control nanoparticles and much better penetration into the target tissue. Gene silencing in primary human TM cells results in a significant reduction of CTGF expression. Thus, HA-coated nanoparticles combined with RNA interference may provide a potential strategy for glaucoma therapy.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | INTRAVITREAL DRUG-DELIVERY; EXTRACELLULAR-MATRIX; HYALURONIC-ACID; OUTFLOW RESISTANCE; ENDOTHELIAL-CELLS; AQUEOUS-HUMOR; ENHANCEMENT; PATHWAYS; FACILITY; MODELS; glaucoma; hyaluronan; layer-by-layer; nanoparticle; siRNA |
| Subjects: | 600 Technology > 610 Medical sciences Medicine 600 Technology > 615 Pharmacy |
| Divisions: | Biology, Preclinical Medicine > Institut für Anatomie > Lehrstuhl für Humananatomie und Embryologie Chemistry and Pharmacy > Institute of Pharmacy > Pharmaceutical Technology (Prof. Göpferich) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 04 Oct 2019 06:39 |
| Last Modified: | 04 Oct 2019 06:39 |
| URI: | https://pred.uni-regensburg.de/id/eprint/13356 |
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