Langgartner, Dominik and Peterlik, Daniel and Foertsch, Sandra and Fuechsl, Andrea M. and Brokmann, Petra and Flor, Peter J. and Shen, Zeli and Fox, James G. and Uschold-Schmidt, Nicole and Lowry, Christopher A. and Reber, Stefan O. (2017) Individual differences in stress vulnerability: The role of gut pathobionts in stress-induced colitis. BRAIN BEHAVIOR AND IMMUNITY, 64. pp. 23-32. ISSN 0889-1591, 1090-2139
Full text not available from this repository. (Request a copy)Abstract
Chronic subordinate colony housing (CSC), an established mouse model for chronic psychosocial stress, promotes a microbial signature of gut inflammation, characterized by expansion of Proteobacteria, specifically Helicobacter spp., in association with colitis development. However, whether the presence of Helicobacter spp. during CSC is critically required for colitis development is unknown. Notably, during previous CSC studies performed at Regensburg University (University 1), male specific-pathogen-free (SPF) CSC mice lived in continuous subordination to a physically present and Helicobacter spp.-positive resident. Therefore, it is likely that CSC mice were colonized, during the CSC procedure, with Helicobacter spp. originating from the dominant resident. In the present study we show that employing SPF CSC mice and Helicobacter spp.-free SPF residents at Ulm University (University 2), results in physiological responses that are typical of chronic psychosocial stress, including increased adrenal and decreased thymus weights, decreased adrenal in vitro adrenocorticotropic hormone (ACTH) responsiveness, and increased anxiety-related behavior. However, in contrast to previous studies that used Helicobacter spp.-positive resident mice, use of Helicobacter spp.-negative resident mice failed to induce spontaneous colitis in SPF CSC mice. Consistent with the hypothesis that the latter is due to a lack of Helicobacter spp. transmission from dominant residents to subordinate mice during the CSC procedure, colonization of SPF residents with Helicobacter typhlonius at University 2, prior to the start of the CSC model, rescued the colitis-inducing potential of CSC exposure. Furthermore, using SPF CSC mice and H. typhlonius-free SPF residents at University 1 prevented CSC-induced colitis. In summary, our data support the hypothesis that the presence or absence of exposure to certain pathobionts contributes to individual variability in susceptibility to stress-/trauma-associated pathologies and to reproducibility of stress-related outcomes between laboratories. (C) 2016 Elsevier Inc. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CHRONIC PSYCHOSOCIAL STRESS; INFLAMMATORY-BOWEL-DISEASE; COLON INFLAMMATION; MALE-MICE; HPA AXIS; HELICOBACTER-TYPHLONIUS; MATERNAL SEPARATION; RESILIENCE; MICROBIOTA; ANXIETY; Chronic psychosocial stress; Chronic subordinate colony housing (CSC); Enterohepatic Helicobacter species (EHS); Inflammatory bowel disease (IBD); Microbiota; Colitis; Stress vulnerability; Stress resilience; Inflammation; Pathobionts |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Biology, Preclinical Medicine > Institut für Physiologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Dec 2018 13:16 |
| Last Modified: | 19 Feb 2019 13:43 |
| URI: | https://pred.uni-regensburg.de/id/eprint/1509 |
Actions (login required)
 |
View Item |
