Kirschbaum-Harriman, Stefanie and Duerkop, Axel and Baeumner, Antje J. (2017) Improving ruthenium-based ECL through nonionic surfactants and tertiary amines. ANALYST, 142 (14). pp. 2648-2653. ISSN 0003-2654, 1364-5528
Full text not available from this repository. (Request a copy)Abstract
The influence of surfactants and coreactants on Ru(bpy)(3)(2+) electrogenerated chemiluminescence (ECL) was investigated comparatively. Specifically, the influence that the two tertiary amines, N-butyldiethanolamine (NBEA) and 2-(dibutylamino) ethanol (DBAE) have on the ECL reaction, alone and in the presence of the two surfactants Triton T X-100 and Zonyl (R) FSN, was investigated, in comparison with that of the well-studied and established coreactant tripropylamine (TPA). Experiments were conducted on poly(methyl methacrylate) substrates coated with plasma-evaporated gold as used in many miniaturized systems. Upon optimization and study of the various combinations, the combination of NBEA/Zonyl FSN provided superior ECL signal characteristics. A limit of detection (LOD) of 2.2 nM Ru(bpy)(3)(2+) was obtained. Compared with the LOD of 0.59 mu M for the commonly used TPA/Triton T X-100 system, the resulting LOD is enhanced by a factor of 250. In addition, significantly more stable signals lead to an increase in sensitivity by a factor of 50. This makes the NBEA/Zonyl (R) FSN system an attractive ECL strategy, especially for miniaturized analytical systems. Furthermore, it became clear that previously postulated effects of surfactants on the enhancement of coreactant-based ECL do not translate to other surfactants and coreactants. We could demonstrate that more complicated mechanisms are at play as the ECL intensity for Ru(bpy)(3)(2+)/ NBEA was the highest in the presence of Zonyl (R) FSN, whereas the ECL signal decreased significantly upon introduction of the surfactant with DBAE as coreactant.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ELECTROGENERATED CHEMILUMINESCENCE; ELECTROCHEMILUMINESCENCE ECL; LIGHT-EMISSION; SYSTEM; TRIS(2,2'-BIPYRIDYL)RUTHENIUM(II); OXIDATION; |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Dec 2018 13:16 |
| Last Modified: | 26 Feb 2019 09:00 |
| URI: | https://pred.uni-regensburg.de/id/eprint/1527 |
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