Uzun, Sema Demirci and Unlu, Naime Akbasoglu and Sendur, Merve and Kanik, Fulya Ekiz and Timur, Suna and Toppare, Levent (2013) A novel promising biomolecule immobilization matrix: Synthesis of functional benzimidazole containing conducting polymer and its biosensor applications. COLLOIDS AND SURFACES B-BIOINTERFACES, 112. pp. 74-80. ISSN 0927-7765, 1873-4367
Full text not available from this repository. (Request a copy)Abstract
In order to construct a robust covalent binding between biomolecule and immobilization platform in biosensor preparation, a novel functional monomer 4-(4,7-di(thiophen-2-yl)-1H-benzo[d]imidazol-2yl)benzaldehyde (BIBA) was designed and successfully synthesized. After electropolymerization of this monomer, electrochemical and spectroelectrochemical properties were investigated in detail. To fabricate the desired biosensor, glucose oxidase (GOx) was immobilized as a model enzyme on the polymer coated graphite electrode with the help of glutaraldehyde (GA). During the immobilization step, an imine bond was formed between the free amino groups of enzyme and aldehyde group of polymer. The surface characterization and morphology were investigated to confirm bioconjugation by X-ray photoelectron spectroscopy (XPS) and transmission electron microscopy (TEM) at each step of biosensor fabrication. The optimized biosensor shows good linearity between 0.02 mM and 1.20 mM and a low limit of detection (LOD) of 2.29 mu M. Kinetic parameters K-m(app) and I-max were determined as 0.94 mM and 10.91 mu A, respectively. The biosensor was tested for human blood serum samples. (C) 2013 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | HYDROGEN-PEROXIDE; GLUCOSE-OXIDASE; COVALENT IMMOBILIZATION; ELECTRODE; FILM; CELL; NANOSTRUCTURES; NANOPARTICLES; DEVICES; SENSORS; Conducting polymer; Biosensor; Enzyme immobilization; Low detection limit; Glucose level |
| Subjects: | 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Chemistry and Pharmacy > Institut für Physikalische und Theoretische Chemie > Chair of Chemistry III - Physical Chemistry (Molecular Spectroscopy and Photochemistry) > Prof. Dr. Hartmut Yersin |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 24 Mar 2020 10:02 |
| Last Modified: | 06 Apr 2020 05:32 |
| URI: | https://pred.uni-regensburg.de/id/eprint/15560 |
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