Cieplik, Fabian and Spaeth, Andreas and Regensburger, Johannes and Gollmer, Anita and Tabenski, Laura and Hiller, Karl-Anton and Baeumler, Wolfgang and Maisch, Tim and Schmalz, Gottfried (2013) Photodynamic biofilm inactivation by SAPYR-An exclusive singlet oxygen photosensitizer. FREE RADICAL BIOLOGY AND MEDICINE, 65. pp. 477-487. ISSN 0891-5849, 1873-4596
Full text not available from this repository. (Request a copy)Abstract
Prevention and control of biofilm-growing microorganisms are serious problems in public health due to increasing resistances of some pathogens against antimicrobial drugs and the potential of these microorganisms to cause severe infections in patients. Therefore, alternative approaches that are capable of killing pathogens are needed to supplement standard treatment modalities. One alternative is the photodynamic inactivation of bacteria (PIB). The lethal effect of PIB is based on the principle that visible light activates a photosensitizer, leading to the formation of reactive oxygen species, e.g., singlet oxygen, which induces phototoxicity immediately during illumination. SAPYR is a new generation of photosensitizers. Based on a 7-perinaphthenone structure, it shows a singlet oxygen quantum yield Phi(Delta) of 99% and is water soluble and photostable. Moreover, it contains a positive charge for good adherence to cell walls of pathogens. In this study, the PIB properties of SAPYR were investigated against monospecies and polyspecies biofilms formed in vitro by oral key pathogens. SAPYR showed a dual mechanism of action against biofilms: (I) it disrupts the structure of the biofilm even without illumination; (II) when irradiated, it inactivates bacteria in a polymicrobial biofilm after one single treatment with an efficacy of >= 99.99%. These results encourage further investigation on the potential of PIB using SAPYR for the treatment of localized infectious diseases. (C) 2013 The Authors. Published by Elsevier Inc. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | IN-VITRO; STAPHYLOCOCCUS-AUREUS; ANTIBIOTIC-RESISTANCE; ANTIMICROBIAL RESISTANCE; BACTERIAL BIOFILMS; MOLECULAR-OXYGEN; DENTAL BIOFILM; CHLORHEXIDINE; THERAPY; SUSCEPTIBILITY; Singlet oxygen; Photodynamic; PIB; Perinaphthenone; Polymicrobial biofilm |
| Subjects: | 500 Science > 540 Chemistry & allied sciences 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Dermatologie und Venerologie Medicine > Lehrstuhl für Zahnerhaltung und Parodontologie > Dr. rer. nat. Karl-Anton Hiller Chemistry and Pharmacy > Institut für Organische Chemie > Lehrstuhl Prof. Dr. Burkhard König |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 24 Mar 2020 10:24 |
| Last Modified: | 06 Apr 2020 05:31 |
| URI: | https://pred.uni-regensburg.de/id/eprint/15565 |
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