Weber-Steffens, Dorothea and Hunold, Katja and Kuerschner, Johanna and Martinez, Sonia Giraldez and Elumalai, Preetham and Schmidt, Dominic and Trevani, Analia and Runza, Valeria L. and Maennel, Daniela N. (2013) Immature mouse granulocytic myeloid cells are characterized by production of ficolin-B. MOLECULAR IMMUNOLOGY, 56 (4). pp. 488-496. ISSN 0161-5890,
Full text not available from this repository. (Request a copy)Abstract
Ficolins activate the lectin pathway of the complement system upon binding to carbohydrate patterns on pathogens. To characterize the producer cells of ficolin-B the expression of mouse ficolin-B, the orthologue of human M-ficolin, was studied in macrophages and dendritic cells during differentiation from bone marrow cells, in primary granulocytes, and during differentiation of granulocytes derived from ER-Hoxb8 cells. Expression of ficolin-B mRNA declined in all myeloid cell types to low levels during terminal differentiation. However, in contrast to macrophages and dendritic cells, ficolin-B expression was enhanced upon activation in granulocytes. High expression of ficolin-B was observed in primary immature neutrophilic CD11b(+) Ly-6C(int) Ly-6G(high) granulocytes when isolated from the bone marrow, in particular during sepsis. Ficolin-B was demonstrated in lysates of primary granulocytes, ER-Hoxb8-derived granulocytes, bone marrow-derived macrophages, and dendritic cells. Native ficolin-B from cell lysates and supernatants of granulocytes activated the lectin pathway as measured by binding to MASP-2 and inducing C4 deposition. Specific staining demonstrated intra-cellular or cell associated ficolin-B protein in activated immature granulocytes deposited in a granular fashion. This study shows that ficolin-B is stored in and set free from immature granulocytic myeloid cells indicating a role in the early infection-induced cellular response of these inflammatory cells. (C) 2013 Elsevier Ltd. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MANNAN-BINDING LECTIN; PATTERN-RECOGNITION MOLECULES; TUMOR-BEARING MICE; COMPLEMENT PATHWAY; SERINE PROTEASE-2; ESCHERICHIA-COLI; SUPPRESSOR-CELLS; NEUTROPHILS; PROTEIN; POLARIZATION; Complement activation; Lectin pathway; Inflammatory cells |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Immunologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 26 Mar 2020 06:31 |
| Last Modified: | 26 Mar 2020 06:31 |
| URI: | https://pred.uni-regensburg.de/id/eprint/15642 |
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