Managh, Amy J. and Edwards, Sheldon L. and Bushell, Andrew and Wood, Kathryn J. and Geissler, Edward K. and Hutchinson, James A. and Hutchinson, Robert W. and Reid, Helen J. and Sharp, Barry L. (2013) Single Cell Tracking of Gadolinium Labeled CD4(+) T Cells by Laser Ablation Inductively Coupled Plasma Mass Spectrometry. ANALYTICAL CHEMISTRY, 85 (22). pp. 10627-10634. ISSN 0003-2700, 1520-6882
Full text not available from this repository. (Request a copy)Abstract
Cellular therapy is emerging as a promising alternative to conventional immunosuppression in the fields of hematopoietic stem cell (HSC) transplantation, autoimmune disease, and solid organ transplantation. Determining the persistence of cell-based therapies in vivo is crucial to understanding their regulatory function and requires the combination of an extremely sensitive detection technique and a stable, long-lifetime cell labeling agent. This paper reports the first application of laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) to perform single cell detection of T cell populations relevant to cellular immunotherapy. Purified human CD4(+) T cells were labeled with commercially available Gd-based magnetic resonance imaging (MRI) contrast agents, Omniscan and Dotarem, which enabled passive loading of up to 10(8) Gd atoms per cell. In mixed preparations of labeled and unlabeled cells, LA-ICP-MS was capable of enumerating labeled cells at close to the predicted ratio. More importantly, LA-ICP-MS single cell analysis demonstrated that the cells retained a sufficient label to remain detectable for up to 10 days post-labeling both in vitro and in vivo in an immunodeficient mouse model.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | NEPHROGENIC SYSTEMIC FIBROSIS; MESENCHYMAL STEM-CELLS; LA-ICP-MS; CLINICAL-APPLICATION; ALLOGRAFT SURVIVAL; REGULATORY CELLS; MRI; NANOPARTICLES; RECIPIENTS; TOLERANCE; |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Chirurgie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 26 Mar 2020 07:47 |
| Last Modified: | 26 Mar 2020 07:47 |
| URI: | https://pred.uni-regensburg.de/id/eprint/15662 |
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