Lituiev, Dmytro S. and Krohn, Nadia G. and Mueller, Bruno and Jackson, David and Hellriegel, Barbara and Dresselhaus, Thomas and Grossniklaus, Ueli (2013) Theoretical and experimental evidence indicates that there is no detectable auxin gradient in the angiosperm female gametophyte. DEVELOPMENT, 140 (22). pp. 4544-4553. ISSN 0950-1991, 1477-9129
Full text not available from this repository. (Request a copy)Abstract
The plant life cycle alternates between a diploid sporophytic and a haploid gametophytic generation. The female gametophyte (FG) of flowering plants is typically formed through three syncytial mitoses, followed by cellularisation that forms seven cells belonging to four cell types. The specification of cell fates in the FG has been suggested to depend on positional information provided by an intrinsic auxin concentration gradient. The goal of this study was to develop mathematical models that explain the formation of this gradient in a syncytium. Two factors were proposed to contribute to the maintenance of the auxin gradient in Arabidopsis FGs: polar influx at early stages and localised auxin synthesis at later stages. However, no gradient could be generated using classical, one-dimensional theoretical models under these assumptions. Thus, we tested other hypotheses, including spatial confinement by the large central vacuole, background efflux and localised degradation, and investigated the robustness of cell specification under different parameters and assumptions. None of the models led to the generation of an auxin gradient that was steep enough to allow sufficiently robust patterning. This led us to re-examine the response to an auxin gradient in developing FGs using various auxin reporters, including a novel degron-based reporter system. In agreement with the predictions of our models, auxin responses were not detectable within the FG of Arabidopsis or maize, suggesting that the effects of manipulating auxin production and response on cell fate determination might be indirect.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CELL-FATE; ARABIDOPSIS-THALIANA; MORPHOGEN GRADIENT; INDOLEACETIC-ACID; PATTERN-FORMATION; EMBRYO SAC; WILD-TYPE; EGG CELL; TRANSPORT; MAIZE; Arabidopsis; Auxin; Female gametophyte; Gradient; Maize; Modelling |
| Subjects: | 500 Science > 580 Botanical sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Pflanzenwissenschaften > Lehrstuhl für Zellbiologie und Pflanzenphysiologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 26 Mar 2020 10:12 |
| Last Modified: | 26 Mar 2020 10:12 |
| URI: | https://pred.uni-regensburg.de/id/eprint/15671 |
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