Klein, Matthias S. and Almstetter, Martin F. and Nuernberger, Nadine and Sigl, Gregor and Gronwald, Wolfram and Wiedemann, Steffi and Dettmer, Katja and Oefner, Peter J. (2013) Correlations between Milk and Plasma Levels of Amino and Carboxylic Acids in Dairy Cows. JOURNAL OF PROTEOME RESEARCH, 12 (11). pp. 5223-5232. ISSN 1535-3893, 1535-3907
Full text not available from this repository. (Request a copy)Abstract
The objective of this study was to investigate the relationship between the concentrations of 19 amino acids, glucose, and seven carboxylic acids in the blood and milk of dairy cows and their correlations with established markers of ketosis. To that end, blood plasma and milk specimens were collected throughout lactation in two breeds of dairy cows of different milk yield. Plasma concentrations of glucose, pyruvate, lactate, alpha-aminobutyrate, beta-hydroxybutyrate (BHBA), and most amino acids, except for glutamate and aspartate, were on average 9.9-fold higher than their respective milk levels. In contrast, glutamate, aspartate, and the Krebs cycle intermediates succinate, fumarate, malate, and citrate were on average 9.1-fold higher in milk than in plasma. For most metabolites, with the exception of BHBA and threonine, no significant correlations were observed between their levels in plasma and milk. Additionally, milk levels of acetone showed significant direct relationships with the glycine-to-alanine ratio and the BHBA concentration in plasma. The marked decline in plasma concentrations of glucose, pyruvate, lactate, and alanine in cows with plasma BHBA levels above the diagnostic cutoff point for subclinical ketosis suggests that these animals fail to meet their glucose demand and, as a consequence, rely increasingly on ketone bodies as a source of energy. The concomitant increase in plasma glycine may reflect not only the excessive depletion of protein reserves but also a potential deficiency of vitamin B-6.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | FREE FATTY-ACIDS; EARLY LACTATION; SUBCLINICAL KETOSIS; REPRODUCTIVE-PERFORMANCE; TRANSITION PERIOD; ENERGY-BALANCE; MAMMARY-GLAND; BLOOD; METABOLISM; VARIABILITY; metabolomics; gas chromatography-mass spectrometry; plasma; milk; dairy cows |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Institut für Funktionelle Genomik > Lehrstuhl für Funktionelle Genomik (Prof. Oefner) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 25 Mar 2020 10:55 |
| Last Modified: | 25 Mar 2020 10:55 |
| URI: | https://pred.uni-regensburg.de/id/eprint/15759 |
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