Wang, Xu-dong and Achatz, Daniela E. and Hupf, Christina and Sperber, Michaela and Wegener, Joachim and Bange, Sebastian and Lupton, John M. and Wolfbeis, Otto S. (2013) Imaging of cellular oxygen via two-photon excitation of fluorescent sensor nanoparticles. SENSORS AND ACTUATORS B-CHEMICAL, 188. pp. 257-262. ISSN 0925-4005,
Full text not available from this repository. (Request a copy)Abstract
Polystyrene nanoparticles (PSNPs) with an average size of 85 nm and loaded with an oxygen-quenchable luminescent ruthenium complex were used to sense and image oxygen inside cells following 2-photon excitation (2-PE). The ruthenium probe possesses a large two-photon absorption cross-section, and 2-PE is achieved by irradiation in the near infrared with commercially available fs-pulsed laser systems. The luminescence of the dye-loaded PSNPs is strongly quenched by oxygen, and Stern-Volmer plots are linear for both conventional single-photon excitation (1-PE) and for 2-PE. The particles do not show any significant cytotoxicity below a threshold concentration of 5 mu g/mL and are readily taken up by mammalian cells (MCF-7), presumably via membrane mediated pathways. Thus, the PSNPs promise to be well suited to image the oxygen distribution in living cells and tissues. The 2-PE is considered to be advantageous over conventional imaging techniques because it works in the near-infrared where background absorption and luminescence of biomatter is much weaker than at excitation wavelengths below 600 nm. (C) 2013 Elsevier B.V. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | COMPLEXES; MICROSCOPY; INDICATORS; CANCER; CELLS; LIGHT; Oxygen sensor; Fluorescence; Two-photon excitation; Quenching; Nanosensor; Intracellular sensing |
| Subjects: | 500 Science > 530 Physics 500 Science > 540 Chemistry & allied sciences |
| Divisions: | Physics > Institute of Experimental and Applied Physics > Chair Professor Lupton > Group John Lupton Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Chemo- und Biosensorik (Prof. Antje J. Bäumner, formerly Prof. Wolfbeis) Chemistry and Pharmacy > Institut für Analytische Chemie, Chemo- und Biosensorik > Bioanalytik und Biosensorik (Prof. Joachim Wegener) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 26 Mar 2020 11:57 |
| Last Modified: | 26 Mar 2020 11:57 |
| URI: | https://pred.uni-regensburg.de/id/eprint/15764 |
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