Scheruebl, Rosmarie and Manns, Detlef and Heilmann, Joerg and Franz, Gerhard (2013) Comparison of HPLC Versus HPTLC-Densitometry for the Quantification of Chromone Glucosides in Saposhnikoviae divaricatae Radix. CHROMATOGRAPHIA, 76 (21-22). pp. 1537-1543. ISSN 0009-5893, 1612-1112
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A HPLC and a HPTLC-densitometric method were developed for the quantification of prim-O-glucosylcimifugin and 4'-O-beta-d-glucosyl-5-O-methylvisamminol the major chromone glucosides in the roots of Saposhnikovia divaricata. The validation of both methods resulted in comparable parameters regarding stability, specificity, linearity, robustness, precision and recovery, whereas complementary advantages were obtained concerning LOD and LOQ. The HPTLC-based densitometry revealed a lower LOD (1.11 versus 4.37 mu g mL(-1) in HPLC) and LOQ (3.36 versus 13.24 mu g mL(-1) in HPLC) for prim-O-glucosylcimifugin, whereas the HPLC resulted in a lower LOD (1.00 versus 4.10 mu g mL(-1) in HPTLC-densitometry) and LOQ (3.04 versus 12.46 mu g mL(-1) in HPTLC-densitometry) for 4'-O-beta-d-glucosyl-5-O-methylvisamminol. Both methods revealed nearly matching contents of the chromones after analysis of different commercially available batches of Saposhnikoviae divaricatae radix with a total content for both chromone glycosides in the range from 0.31 +/- A 0.011 to 0.56 +/- A 0.021 % determined by HPLC and between 0.34 +/- A 0.011 and 0.61 +/- A 0.009 % determined by HPTLC. The plant material cultivated in Germany showed a very similar content and ratio of both chromone glucosides in comparison to the standard batches originating from China.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | LIQUID-CHROMATOGRAPHY; QUANTITATIVE-ANALYSIS; MASS-SPECTROMETRY; HPTLC-densitometry; HPLC; Saposhnikovia divaricata; Traditional Chinese medicine; Chromone glucosides; Quality control |
| Subjects: | 600 Technology > 615 Pharmacy |
| Divisions: | Chemistry and Pharmacy > Institute of Pharmacy > Pharmaceutical Biology (Prof. Heilmann) Chemistry and Pharmacy > Institute of Pharmacy > Alumni or Retired Professors > Prof. Franz |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 01 Apr 2020 10:53 |
| Last Modified: | 01 Apr 2020 10:53 |
| URI: | https://pred.uni-regensburg.de/id/eprint/15783 |
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