Validation of an rpoB Gene PCR Assay for Detection of Tropheryma whipplei: 10 Years' Experience in a National Reference Laboratory

Moter, Annette and Schmiedel, Dinah and Petrich, Annett and Wiessner, Alexandra and Kikhney, Judith and Schneider, Thomas and Moos, Verena and Goebel, Ulf B. and Reischl, Udo (2013) Validation of an rpoB Gene PCR Assay for Detection of Tropheryma whipplei: 10 Years' Experience in a National Reference Laboratory. JOURNAL OF CLINICAL MICROBIOLOGY, 51 (11). pp. 3858-3861. ISSN 0095-1137, 1098-660X

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Abstract

The performance of a real-time PCR assay targeting the Tropheryma whipplei rpoB gene was evaluated using test strains and 1,236 clinical specimens in a national reference laboratory. The novel rpoB-PCR assay proved to be specific, revealed improved analytical sensitivity, and substantially accelerated detection of T. whipplei DNA in clinical specimens.

Item Type: Article
Uncontrolled Keywords: REAL-TIME PCR; CLINICAL SPECIMENS; DISEASE; WHIPPELII; SEQUENCES; DNA;
Subjects: 600 Technology > 610 Medical sciences Medicine
Divisions: Medicine > Lehrstuhl für Medizinische Mikrobiologie und Hygiene
Depositing User: Dr. Gernot Deinzer
Date Deposited: 01 Apr 2020 11:00
Last Modified: 01 Apr 2020 11:00
URI: https://pred.uni-regensburg.de/id/eprint/15788

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