Lidsky, Peter V. and Sprenger, Frank and Lehner, Christian F. (2013) Distinct modes of centromere protein dynamics during cell cycle progression in Drosophila S2R+cells. JOURNAL OF CELL SCIENCE, 126 (20). pp. 4782-4793. ISSN 0021-9533, 1477-9137
Full text not available from this repository. (Request a copy)Abstract
Centromeres are specified epigenetically in animal cells. Therefore, faithful chromosome inheritance requires accurate maintenance of epigenetic centromere marks during progression through the cell cycle. Clarification of the mechanisms that control centromere protein behavior during the cell cycle should profit from the relatively simple protein composition of Drosophila centromeres. Thus we have analyzed the dynamics of the three key players Cid/Cenp-A, Cenp-C and Cal1 in S2R+ cells using quantitative microscopy and fluorescence recovery after photobleaching, in combination with novel fluorescent cell cycle markers. As revealed by the observed protein abundances and mobilities, centromeres proceed through at least five distinct states during the cell cycle, distinguished in part by unexpected Cid behavior. In addition to the predominant Cid loading onto centromeres during G1, a considerable but transient increase was detected during early mitosis. A low level of Cid loading was detected in late S and G2, starting at the reported time of centromere DNA replication. Our results reveal the complexities of Drosophila centromere protein dynamics and its intricate coordination with cell cycle progression.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CENP-A NUCLEOSOMES; HISTONE H3 VARIANT; E3 UBIQUITIN LIGASE; IN-VIVO; BUDDING YEAST; S2 CELLS; KINETOCHORE FORMATION; CHECKPOINT CONTROL; NONHISTONE SCM3; LIVING CELLS; Centromere; Cid; Cenp-A; Cenp-C; Cal1; Cell cycle; Mitosis; S phase; Drosophila |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Cell Cycle Control > Prof. Dr. Frank Sprenger |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 30 Mar 2020 13:43 |
| Last Modified: | 30 Mar 2020 13:43 |
| URI: | https://pred.uni-regensburg.de/id/eprint/15843 |
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