Neumeier, Markus and Krautbauer, Sabrina and Schmidhofer, Sandra and Hader, Yvonne and Eisinger, Kristina and Eggenhofer, Elke and Froehner, Stanley C. and Adams, Marvin E. and Mages, Wolfgang and Buechler, Christa (2013) Adiponectin receptor 1 C-terminus interacts with PDZ-domain proteins such as syntrophins. EXPERIMENTAL AND MOLECULAR PATHOLOGY, 95 (2). pp. 180-186. ISSN 0014-4800, 1096-0945
Full text not available from this repository. (Request a copy)Abstract
Adiponectin receptor 1 (AdipoR1) is one of the two signaling receptors of adiponectin with multiple beneficial effects in metabolic diseases. AdipoR1 C-terminal peptide is concordant with the consensus sequence of class I PSD-95, disc large, ZO-1 (PDZ) proteins, and screening of a liver yeast two hybrid library identified binding to beta 2-syntrophin (SNTB2). Hybridization of a PDZ-domain array with AdipoR1 C-terminal peptide shows association with PDZ-domains of further proteins including beta 1- and alpha-syntrophin (SNTA). Interaction of PDZ proteins and C-terminal peptides requires a free carboxy terminus next to the PDZ-binding region and is blocked by carboxy terminal added tags. N-terminal tagged AdipoR1 is more highly expressed than C-terminal tagged receptor suggesting that the free carboxy terminus may form a complex with PDZ proteins to regulate cellular AdipoR1 levels. The C- and 4-terminal tagged AdipoR1 proteins are mainly localized in the cytoplasma. N-terminal but not C-terminal tagged AdipoR1 colocalizes with syntrophins in adiponectin incubated Huh7 cells. Adiponectin induced hepatic phosphorylation of AMPK and p38 MAPK which are targets of AdipoR1 is, however, not blocked in SNTA and SNTB2 deficient mice. Further, AdipoR1 protein is similarly abundant in the liver of knock-out and wild type mice when kept on a standard chow or a high fat diet. In summary these data suggest that AdipoR1 protein levels are regulated by so far uncharacterized class I PDZ proteins which are distinct from SNTA and SNTB2. (c) 2013 Elsevier Inc. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | CASSETTE TRANSPORTER A1; BINDING-PROTEINS; CELL-SURFACE; KINASE CK2; ACTIVATION; EXPRESSION; APPL1; DIMERIZATION; PATHWAYS; REVEALS; Adiponectin; PDZ-protein; Hepatocyte; Syntrophin |
| Subjects: | 500 Science > 570 Life sciences 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin I Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Cell Cycle Control > Prof. Dr. Wolfgang Seufert |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 30 Mar 2020 07:29 |
| Last Modified: | 30 Mar 2020 07:29 |
| URI: | https://pred.uni-regensburg.de/id/eprint/15933 |
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