Schweikl, Helmut and Hiller, Karl-Anton and Carl, Ulrich and Schweiger, Rainer and Eidt, Andreas and Ruhl, Stefan and Mueller, Rainer and Schmalz, Gottfried (2013) Salivary protein adsorption and Streptococccus gordonii adhesion to dental material surfaces. DENTAL MATERIALS, 29 (10). pp. 1080-1089. ISSN 0109-5641,
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Objectives. The initial adhesion of microorganisms to clinically used dental biomaterials is influenced by physico-chemical parameters like hydrophobicity and pre-adsorption of salivary proteins. Here, polymethyl methacrylate (PMMA), polyethylene (PE), polytetrafluoroethylene (PTFE), silicone (Mucopren soft), silorane-based (Filtek Silorane) and methacrylate-based (Tetric EvoCeram) dental composites, a conventional glassionomer cement as well as cobalt-chromium-molybdenum (Co28Cr6Mo) and titanium (Ti6Al4V) were tested for adsorption of salivary proteins and adhesion of Streptococcus gordonii DL1. Methods. Wettability of material surfaces precoated with salivary proteins or left in phosphate-buffered saline was determined by the measurement of water contact angles. Amounts of adsorbed proteins were determined directly on material surfaces after biotinylation of amino groups and detection by horseradish peroxidase-conjugated avidin-D. The same technique was used to analyze for the binding of biotinylated bacteria to material surfaces. Results. The highest amount of proteins (0.18 mu g/cm(2)) adsorbed to hydrophobic PTFE samples, and the lowest amount (0.025 mu g/cm(2)) was detected on silicone. The highest number of S. gordonii (3.2 x 10(4) CFU/mm(2)) adhered to the hydrophilic glassionomer cement surface coated with salivary proteins, and the lowest number (4 x 10(3) CFU/mm(2)) was found on the hydrophobic silorane-based composite. Hydrophobicity of pure material surfaces and the number of attached microorganisms were weakly negatively correlated. No such correlation between hydrophobicity and the number of bacteria was detected when surfaces were coated with salivary proteins. Significance. Functional groups added by the adsorption of specific salivary proteins to material surfaces are more relevant for initial bacterial adhesion than hydrophobicity as a physical property. (C) 2013 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | BACTERIAL ADHESION; IN-VITRO; BIOMATERIAL SURFACES; BIOFILM FORMATION; IONIC-STRENGTH; MUTANS; MECHANISMS; BINDING; FORCES; RESIN; Dental materials; Protein absorption; Bacterial adhesion; Saliva |
| Subjects: | 500 Science > 540 Chemistry & allied sciences 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Zahnerhaltung und Parodontologie > Prof. Dr. rer. nat. Helmut Schweikl Medicine > Lehrstuhl für Zahnerhaltung und Parodontologie > Dr. rer. nat. Karl-Anton Hiller Chemistry and Pharmacy > Institut für Physikalische und Theoretische Chemie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 31 Mar 2020 11:08 |
| Last Modified: | 31 Mar 2020 11:08 |
| URI: | https://pred.uni-regensburg.de/id/eprint/15998 |
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