Elling, Felix J. and Koenneke, Martin and Nicol, Graeme W. and Stieglmeier, Michaela and Bayer, Barbara and Spieck, Eva and de la Torre, Jose R. and Becker, Kevin W. and Thomm, Michael and Prosser, James I. and Herndl, Gerhard J. and Schleper, Christa and Hinrichs, Kai-Uwe (2017) Chemotaxonomic characterisation of the thaumarchaeal lipidome. ENVIRONMENTAL MICROBIOLOGY, 19 (7). pp. 2681-2700. ISSN 1462-2912, 1462-2920
Full text not available from this repository. (Request a copy)Abstract
Thaumarchaeota are globally distributed and abundant microorganisms occurring in diverse habitats and thus represent a major source of archaeal lipids. The scope of lipids as taxonomic markers in microbial ecological studies is limited by the scarcity of comparative data on the membrane lipid composition of cultivated representatives, including the phylum Thaumarchaeota. Here, we comprehensively describe the core and intact polar lipid (IPL) inventory of ten ammonia-oxidising thaumarchaeal cultures representing all four characterized phylogenetic clades. IPLs of these thaumarchaeal strains are generally similar and consist of membrane-spanning, glycerol dibiphytanyl glycerol tetraethers with monoglycosyl, diglycosyl, phosphohexose and hexose-phosphohexose headgroups. However, the relative abundances of these IPLs and their core lipid compositions differ systematically between the phylogenetic subgroups, indicating high potential for chemotaxonomic distinction of thaumarchaeal clades. Comparative lipidomic analyses of 19 euryarchaeal and crenarchaeal strains suggested that the lipid methoxy archaeol is synthesized exclusively by Thaumarchaeota and may thus represent a diagnostic lipid biomarker for this phylum. The unprecedented diversity of the thaumarchaeal lipidome with 118 different lipids suggests that membrane lipid composition and adaptation mechanisms in Thaumarchaeota are more complex than previously thought and include unique lipids with as yet unresolved properties.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | AMMONIA-OXIDIZING ARCHAEA; DIALKYL GLYCEROL TETRAETHERS; OXYGEN MINIMUM ZONE; HIGH-MOUNTAIN LAKES; INTACT POLAR; MEMBRANE-LIPIDS; ETHER LIPIDS; NITROSOPUMILUS-MARITIMUS; MICROBIAL ECOLOGY; WATER COLUMN; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) > Prof. Dr. Michael Thomm |
| Depositing User: | Petra Gürster |
| Date Deposited: | 14 Dec 2018 13:16 |
| Last Modified: | 10 Sep 2020 09:34 |
| URI: | https://pred.uni-regensburg.de/id/eprint/1600 |
Actions (login required)
 |
View Item |
