Redondo, Beatriz Blanco and Bunz, Melanie and Halder, Partho and Sadanandappa, Madhumala K. and Muehlbauer, Barbara and Erwin, Felix and Hofbauer, Alois and Rodrigues, Veronica and VijayRaghavan, K. and Ramaswami, Mani and Rieger, Dirk and Wegener, Christian and Foerster, Charlotte and Buchner, Erich (2013) Identification and Structural Characterization of Interneurons of the Drosophila Brain by Monoclonal Antibodies of the Wurzburg Hybridoma Library. PLOS ONE, 8 (9): e75420. ISSN 1932-6203,
Full text not available from this repository. (Request a copy)Abstract
Several novel synaptic proteins have been identified by monoclonal antibodies (mAbs) of the Wurzburg hybridoma library generated against homogenized Drosophila brains, e.g. cysteine string protein, synapse-associated protein of 47 kDa, and Bruchpilot. However, at present no routine technique exists to identify the antigens of mAbs of our library that label only a small number of cells in the brain. Yet these antibodies can be used to reproducibly label and thereby identify these cells by immunohistochemical staining. Here we describe the staining patterns in the Drosophila brain for ten mAbs of the Wurzburg hybridoma library. Besides revealing the neuroanatomical structure and distribution of ten different sets of cells we compare the staining patterns with those of antibodies against known antigens and GFP expression patterns driven by selected Gal4 lines employing regulatory sequences of neuronal genes. We present examples where our antibodies apparently stain the same cells in different Gal4 lines suggesting that the corresponding regulatory sequences can be exploited by the split-Gal4 technique for transgene expression exclusively in these cells. The detection of Gal4 expression in cells labeled by mAbs may also help in the identification of the antigens recognized by the antibodies which then in addition to their value for neuroanatomy will represent important tools for the characterization of the antigens. Implications and future strategies for the identification of the antigens are discussed.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PIGMENT-DISPERSING HORMONE; NERVOUS-SYSTEM; IMMUNOREACTIVE NEURONS; VISUAL-SYSTEM; OPTIC LOBE; MELANOGASTER; PROTEIN; LAMINA; GENE; |
| Subjects: | 500 Science > 590 Zoological sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Zoologie > Entwicklungsbiologie (Prof. Dr. Stephan Schneuwly) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 31 Mar 2020 12:33 |
| Last Modified: | 31 Mar 2020 12:34 |
| URI: | https://pred.uni-regensburg.de/id/eprint/16028 |
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