Shen-Miller, J. and Lindner, Petra and Xie, Yongming and Villa, Sarah and Wooding, Kerry and Clarke, Steven G. and Loo, Rachel R. O. and Loo, Joseph A. (2013) Thermal-Stable Proteins of Fruit of Long-Living Sacred Lotus Nelumbo nucifera Gaertn var. China Antique. TROPICAL PLANT BIOLOGY, 6 (2-3). pp. 69-84. ISSN 1935-9756, 1935-9764
Full text not available from this repository. (Request a copy)Abstract
Single-seeded fruit of the sacred lotus Nelumbo nucifera Gaertn var. China Antique from NE China have been shown to remain viable for as long as similar to 1,300 years, determined by direct radiocarbon-dating, and to have a germination rate of 84 %. The pericarp, a fruit tissue that encloses the single seeds of Nelumbo, is one of the major factors contributing to fruit longevity. Proteins that are heat stable and have a protective function are equally important to such centuries-long seed viability. We document proteins of Nelumbo fruit that are able to withstand heating, 32 % of which remained soluble in the 110 degrees C-treated embryo axis of a 549-year-old fruit and 76 % retained fluidity in its cotyledons. The genome of Nelumbo has recently been published and annotated. The amino-acid sequences of 11 "thermal proteins" (soluble at 100 degrees C) of modern Nelumbo embryo axes and cotyledons, identified by mass spectrometry, Western blot and bioassay, are assembled and aligned with those of an archaeal hyperthermophile Methancaldococcus jannaschii ("Mj," an anaerobic methanogen having a growth optimum of 85 degrees C) and with those of five mesophile angiosperms. These thermal proteins have roles in protection and repair under stress. More than half (55 %) of the durable Nelumbo thermal proteins are present in the archaean Mj, indicating their ancient history. One Nelumbo protein-repair enzyme exhibits activity at 100 degrees C, having a heat-tolerance higher than the comparable enzyme of Arabidopsis. A list of 30 sequenced but unassembled thermal proteins of Nelumbo is appended.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | L-ISOASPARTYL METHYLTRANSFERASE; METHANOCOCCUS-JANNASCHII; POLYACRYLAMIDE-GELS; OXIDATIVE STRESS; FUNCTIONAL-CHARACTERIZATION; ELONGATION FACTOR-1-ALPHA; HYPERTHERMOPHILIC ENZYMES; ELECTROPHORETIC TRANSFER; SUPEROXIDE-DISMUTASE; HIGHER-PLANTS; Nelumbo nucifera China antique; Heat-soluble (100 degrees C) Proteins; Hyperthermophile-Mesophile protein-alignments; Stress-and-repair thermoproteins |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 31 Mar 2020 14:04 |
| Last Modified: | 31 Mar 2020 14:04 |
| URI: | https://pred.uni-regensburg.de/id/eprint/16057 |
Actions (login required)
 |
View Item |
