Diermeier, Sarah D. and Nemeth, Attila and Rehli, Michael and Grummt, Ingrid and Laengst, Gernot (2013) Chromatin-Specific Regulation of Mammalian rDNA Transcription by Clustered TTF-I Binding Sites. PLOS GENETICS, 9 (9): e1003786. ISSN 1553-7404,
Full text not available from this repository. (Request a copy)Abstract
Enhancers and promoters often contain multiple binding sites for the same transcription factor, suggesting that homotypic clustering of binding sites may serve a role in transcription regulation. Here we show that clustering of binding sites for the transcription termination factor TTF-I downstream of the pre-rRNA coding region specifies transcription termination, increases the efficiency of transcription initiation and affects the three-dimensional structure of rRNA genes. On chromatin templates, but not on free rDNA, clustered binding sites promote cooperative binding of TTF-I, loading TTF-I to the downstream terminators before it binds to the rDNA promoter. Interaction of TTF-I with target sites upstream and downstream of the rDNA transcription unit connects these distal DNA elements by forming a chromatin loop between the rDNA promoter and the terminators. The results imply that clustered binding sites increase the binding affinity of transcription factors in chromatin, thus influencing the timing and strength of DNA-dependent processes.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | RIBOSOMAL-RNA GENES; COOPERATIVE DNA-BINDING; REMODELING COMPLEX NORC; POLYMERASE-I; SYNERGISTIC ACTIVATION; HISTONE MODIFICATIONS; FUNCTIONAL BINDING; DROSOPHILA GENOME; TERMINATION; PROMOTER; |
| Subjects: | 500 Science > 570 Life sciences 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Innere Medizin III (Hämatologie und Internistische Onkologie) Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie III > Prof. Dr. Gernot Längst |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 01 Apr 2020 06:42 |
| Last Modified: | 01 Apr 2020 06:42 |
| URI: | https://pred.uni-regensburg.de/id/eprint/16116 |
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