Berner, A. and Henkel, J. and Woodruff, M. A. and Saifzadeh, S. and Kirby, G. and Zaiss, S. and Gohlke, J. and Reichert, J. C. and Nerlich, M. and Schuetz, M. A. and Hutmacher, D. W. (2017) Scaffold-cell bone engineering in a validated preclinical animal model: precursors vs differentiated cell source. JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE, 11 (7). pp. 2081-2089. ISSN 1932-6254, 1932-7005
Full text not available from this repository. (Request a copy)Abstract
The properties of osteoblasts (OBs) isolated from the axial skeleton (tOBs) differ from OBs of the orofacial skeleton (mOBs) due to the different embryological origins of the bones. The aim of the study was to assess and compare the regenerative potential of allogenic bone marrow-derived mesenchymal progenitor cells with allogenic tOBs and allogenic mOBs in combination with a mPCL-TCP scaffold in critical-sized segmental bone defects in sheep tibiae. After 6months, the tibiae were explanted and underwent biomechanical testing, micro-computed tomography (microCT) and histological and immunohistochemical analyses. Allogenic MPCs demonstrated a trend towards a better outcome in biomechanical testing and the mean values of newly formed bone. Biomechanical, microCT and histological analysis showed no significant differences in the bone regeneration potential of tOBs and mOBs in our in vitro study, as well as in the bone regeneration potential of different cell types in vivo. Copyright (c) 2015 John Wiley & Sons, Ltd.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MESENCHYMAL STEM-CELLS; IMMUNOLOGICAL-PROPERTIES; PROGENITOR CELLS; REGENERATION; CREST; BIOLOGY; GRAFTS; SHEEP; FATE; bone defect; mesenchymal progenitor cells; allogenic cells; osteoblasts; bone tissue engineering; bone regeneration; scaffolds; polycaprolactone |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Unfallchirurgie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Dec 2018 13:16 |
| Last Modified: | 13 Feb 2019 09:01 |
| URI: | https://pred.uni-regensburg.de/id/eprint/1616 |
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