Weide, Thomas and Vollenbroeker, Beate and Schulze, Ulf and Djuric, Ivona and Edeling, Maria and Bonse, Jakob and Hochapfel, Florian and Panichkina, Olga and Wennmann, Dirk-Oliver and George, Britta and Kim, Seonhee and Daniel, Christoph and Seggewiss, Jochen and Amann, Kerstin and Kriz, Wilhelm and Krahn, Michael P. and Pavenstaedt, Hermann (2017) Pals1 Haploinsufficiency Results in Proteinuria and Cyst Formation. JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY, 28 (7). pp. 2093-2107. ISSN 1046-6673, 1533-3450
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The nephron is the basic physiologic subunit of the mammalian kidney and is made up of several apicobasally polarized epithelial cell types. The process of apicobasal polarization in animal cells is controlled by the evolutionarily conserved Crumbs (CRB), Partitioning-defective, and Scribble protein complexes. Here, we investigated the role of protein associated with LIN-7 1 (Pals1, also known as Mpp5), a core component of the apical membrane-determining CRB complex in the nephron. Pals1 interacting proteins, including Crb3 andWwtr1/Taz, have been linked to renal cyst formation in mice before. Immunohistologic analysis revealed Pals1 expression in renal tubular cells and podocytes of human kidneys. Mice lacking one Pals1 allele (functionally haploid for Pals1) in nephrons developed a fully penetrant phenotype, characterized by cyst formation and severe defects in renal barrier function, which led to death within 6-8 weeks. In Drosophila nephrocytes, deficiency of the Pals1 ortholog caused alterations in slit-diaphragm-like structures. Additional studies in epithelial cell culture models revealed that Pals1 functions as a dose-dependent upstream regulator of the crosstalk between Hippo-and TGF-beta-mediated signaling. Furthermore, Pals1 haploinsufficiency in mouse kidneys associated with the upregulation of Hippo pathway target genes and marker genes of TGF-beta signaling, including biomarkers of renal diseases. These findings support a link between apical polarity proteins and renal diseases, especially renal cyst diseases. Further investigation of the Pals1-linked networks is required to decipher the mechanisms underlying the pathogenesis of these diseases.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | MAMMALIAN EPITHELIAL-CELLS; HIPPO PATHWAY; POLARITY COMPLEXES; KIDNEY DEVELOPMENT; TIGHT JUNCTION; SLIT DIAPHRAGM; IN-VIVO; DROSOPHILA; CRUMBS; MORPHOGENESIS; |
| Subjects: | 500 Science > 570 Life sciences 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Biology, Preclinical Medicine > Institut für Anatomie > Lehrstuhl für Molekulare und zelluläre Anatomie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 14 Dec 2018 13:16 |
| Last Modified: | 28 Feb 2019 11:09 |
| URI: | https://pred.uni-regensburg.de/id/eprint/1621 |
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