Fouqueau, Thomas and Zeller, Mirijam E. and Cheung, Alan C. and Cramer, Patrick and Thomm, Michael (2013) The RNA polymerase trigger loop functions in all three phases of the transcription cycle. NUCLEIC ACIDS RESEARCH, 41 (14). pp. 7048-7059. ISSN 0305-1048,
Full text not available from this repository. (Request a copy)Abstract
The trigger loop (TL) forms a conserved element in the RNA polymerase active centre that functions in the elongation phase of transcription. Here, we show that the TL also functions in transcription initiation and termination. Using recombinant variants of RNA polymerase from Pyrococcus furiosus and a reconstituted transcription system, we demonstrate that the TL is essential for initial RNA synthesis until a complete DNA-RNA hybrid is formed. The archaeal TL is further important for transcription fidelity during nucleotide incorporation, but not for RNA cleavage during proofreading. A conserved glutamine residue in the TL binds the 2'-OH group of the nucleoside triphosphate (NTP) to discriminate NTPs from dNTPs. The TL also prevents aberrant transcription termination at non-terminator sites.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | STRUCTURAL BASIS; ARCHAEAL TRANSCRIPTION; ELONGATION COMPLEXES; ANGSTROM RESOLUTION; INITIATION COMPLEX; II TRANSCRIPTION; ACTIVE-CENTER; IN-VITRO; TERMINATION; MECHANISM; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) > Prof. Dr. Michael Thomm |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 03 Apr 2020 13:15 |
| Last Modified: | 03 Apr 2020 13:15 |
| URI: | https://pred.uni-regensburg.de/id/eprint/16286 |
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