Ferstl, Matthias and Drechsler, Markus and Rachel, Reinhard and Rischer, Matthias and Engel, Juergen and Backofen, Michael and Goepferich, Achim (2013) The impact of polyelectrolyte structure on the shape of nanoassemblies with cationic peptides. JOURNAL OF PHARMACEUTICAL SCIENCES, 102 (8). pp. 2599-2607. ISSN 0022-3549,
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We investigated how the structure of nanofibers, resulting from interactions between anionic polyelectrolytes and cationic peptides, relies on the properties of the polyelectrolyte component. By using hyaluronate (H), carboxymethylcellulose (CMC), xanthan (X), and ozarelix (O), a cationic decapeptide, we determined the influence of characteristic polyelectrolyte parameters such as size and charge density on the formation of polyelectrolyte-peptide complexes. Transmission electron microscopy of unstained, frozen hydrated, or negatively stained samples revealed that the interaction between different anionic polyelectrolytes and ozarelix led to the formation of distinctly shaped nanofibers. CMC formed rather flexible structures with alternating thin and thick segments within the nanofibers with diameters ranging from 10 to 16nm and a length of up to 1m. Hyaluronate, a high-molecular-mass molecule, formed extra-long aggregates of more than 5m. Individual fibers with a diameter of 8nm aggregated to bigger strands. The nonlinear polysaccharide xanthan gum led to highly coiled structures. The diameter of the respective nanofibers varied between 15 and 25nm. Isothermal titration calorimetry was used to determine the binding constants and the thermodynamic parameters of the different polyelectrolyte-peptide complexes. The binding constant, which was of the order of 10(6) M-1, indicated a strong binding affinity, but also showed differences among the polyelectrolytes. These differences might be useful for prospective applications as drug delivery systems. (c) 2013 Wiley Periodicals, Inc. and the American Pharmacists Association J Pharm Sci 102:2599-2607, 2013
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | POLYMER NANOFIBERS; XANTHAN GUM; MOLECULAR-WEIGHT; HYALURONIC-ACID; NANOCOMPOSITES; MICROSCOPY; DELIVERY; RELEASE; BINDING; calorimetry (ITC); protein formulation; imaging methods; nanotechnology; polyelectrolytes; physical characterization; particle size |
| Subjects: | 500 Science > 570 Life sciences 600 Technology > 615 Pharmacy |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Mikrobiologie (Archaeenzentrum) > Prof. Dr. Reinhard Rachel Chemistry and Pharmacy > Institute of Pharmacy > Pharmaceutical Technology (Prof. Göpferich) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 03 Apr 2020 13:24 |
| Last Modified: | 03 Apr 2020 13:24 |
| URI: | https://pred.uni-regensburg.de/id/eprint/16291 |
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