Ignatov, Tanja and Modl, Saskia and Thulig, Maike and Weissenborn, Christine and Treeck, Oliver and Ortmann, Olaf and Zenclussen, A. C. and Costa, Serban Dan and Kalinski, Thomas and Ignatov, Atanas (2013) GPER-1 acts as a tumor suppressor in ovarian cancer. JOURNAL OF OVARIAN RESEARCH, 6: UNSP 51. ISSN 1757-2215,
Full text not available from this repository. (Request a copy)Abstract
Background: It is known that the new membrane-bound estrogen receptor GPER-1 acts suppressive in breast cancer cells and its expression decreases during disease progression. This study was conducted to evaluate the GPER-1 expression in ovarian cancer and its correlation with progression. Its function was tested in vitro in ovarian cancer cells. Patients and methods: GPER-1 expression was analyzed by immunohistochemistry in 35 benign ovarian tumors, 35 tumors of low-malignant potential and in 124 ovarian cancers. GPER-1 expression was correlated to the prospectively evaluated disease-free survival of ovarian cancer patients. We also tested GPER-1 expression in ovarian cancer cells and the effect of GPER-1 stimulation on cell growth. Results: GPER-1 expression was significantly lower in ovarian cancer tissue than in benign and low-malignant ovarian tumors. GPER-1 expression was observed in 83.1% of malignant tumors and was higher in early stage cancers and tumors with high histological differentiation. GPER-1 expression was associated with favourable clinical outcome. The difference in 2-year disease-free survival by GPER-1 expression was significant, 28.6% for GPER-1 negative and 59.2% for GPER-1 positive cases (p = 0.002). GPER-1 expression was observed in SKOV-3 and OVCAR-3 ovarian cancer cell lines. G-1, a selective GPER-1 agonist, suppressed proliferation of the two cell types via inhibition of cell cycle progression in G2/M phase and stimulation of caspase-dependent apoptosis. The blockade in G2/M phase was associated with increased expression of cyclin B1 and Cdc2 and phosphorylation of histone 3. Conclusion: GPER-1 emerges as a new tumor suppressor with unsuspected therapeutic potential for ovarian cancer.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PROTEIN-COUPLED RECEPTOR; GROWTH-FACTOR RECEPTOR; BREAST-CANCER; CELL-CYCLE; GPR30; ESTROGEN; PROLIFERATION; EXPRESSION; SURVIVAL; G-1; GPR30; GPER-1; Ovarian cancer |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Frauenheilkunde und Geburtshilfe (Schwerpunkt Frauenheilkunde) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 06 Apr 2020 08:53 |
| Last Modified: | 06 Apr 2020 08:53 |
| URI: | https://pred.uni-regensburg.de/id/eprint/16368 |
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