Ohmayer, Uli and Gamalinda, Michael and Sauert, Martina and Ossowski, Julius and Poell, Gisela and Linnemann, Jan and Hierlmeier, Thomas and Perez-Fernandez, Jorge and Kumcuoglu, Beril and Leger-Silvestre, Isabelle and Faubladier, Marlene and Griesenbeck, Joachim and Woolford, John and Tschochner, Herbert and Milkereit, Philipp (2013) Studies on the Assembly Characteristics of Large Subunit Ribosomal Proteins in S. cerevisae. PLOS ONE, 8 (7): UNSP e6841. ISSN 1932-6203,
Full text not available from this repository. (Request a copy)Abstract
During the assembly process of ribosomal subunits, their structural components, the ribosomal RNAs (rRNAs) and the ribosomal proteins (r-proteins) have to join together in a highly dynamic and defined manner to enable the efficient formation of functional ribosomes. In this work, the assembly of large ribosomal subunit (LSU) r-proteins from the eukaryote S. cerevisiae was systematically investigated. Groups of LSU r-proteins with specific assembly characteristics were detected by comparing the protein composition of affinity purified early, middle, late or mature LSU (precursor) particles by semi-quantitative mass spectrometry. The impact of yeast LSU r-proteins rpL25, rpL2, rpL43, and rpL21 on the composition of intermediate to late nuclear LSU precursors was analyzed in more detail. Effects of these proteins on the assembly states of other r-proteins and on the transient LSU precursor association of several ribosome biogenesis factors, including Nog2, Rsa4 and Nop53, are discussed.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | ESCHERICHIA-COLI RIBOSOMES; YEAST SACCHAROMYCES-CEREVISIAE; NUCLEAR EXPORT RECEPTOR; ANGSTROM RESOLUTION; TRANSLATION INITIATION; BIOGENESIS FACTORS; 50S SUBUNIT; RNA; MATURATION; BINDING; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie III > Prof. Dr. Herbert Tschochner Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie III > Dr. Joachim Griesenbeck Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie III > Dr. Philipp Milkereit |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 06 Apr 2020 09:27 |
| Last Modified: | 06 Apr 2020 09:27 |
| URI: | https://pred.uni-regensburg.de/id/eprint/16369 |
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