Duttenhoefer, F. and de Freitas, R. Lara and Meury, T. and Loibl, M. and Benneker, L. M. and Richards, R. G. and Alini, M. and Verrier, S. (2013) 3D SCAFFOLDS CO-SEEDED WITH HUMAN ENDOTHELIAL PROGENITOR AND MESENCHYMAL STEM CELLS: EVIDENCE OF PREVASCULARISATION WITHIN 7 DAYS. EUROPEAN CELLS & MATERIALS, 26. pp. 49-65. ISSN 1473-2262,
Full text not available from this repository. (Request a copy)Abstract
Blood supply is a critical issue in most tissue engineering approaches for large defect healing. As vessel ingrowth from surrounding tissues is proven to be insufficient, current strategies are focusing on the neo-vascularisation process. In the present study, we developed an in vitro pre-vascularised construct using 3D polyurethane (PU) scaffolds, based on the association of human Endothelial Progenitor Cells (EPC, CD34+ and CD133+) with human Mesenchymal Stem Cells (MSC). We showed the formation of luminal tubular structures in the co-seeded scaffolds as early as day 7 in culture. These tubular structures were proven positive for endothelial markers von Willebrand Factor and PECAM-1. Of special significance in our constructs is the presence of CD146-positive cells, as a part of the neovasculature scaffolding. These cells, coming from the mesenchymal stem cells population (MSC or EPC-depleted MSC), also expressed other markers of pericyte cells (NG2 and alpha SMA) that are known to play a pivotal function in the stabilisation of newly formed pre-vascular networks. In parallel, in co-cultures, osteogenic differentiation of MSCs occurred earlier when compared to MSCs monocultures, suggesting the close cooperation between the two cell populations. The presence of angiogenic factors (from autologous platelet lysates) in association with osteogenic factors seems to be crucial for both cell populations' cooperation. These results are promising for future clinical applications, as all components (cells, growth factors) can be prepared in an autologous way.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | HUMAN PERIPHERAL-BLOOD; HUMAN BONE-MARROW; IN-VITRO; GROWTH-FACTOR; POLYURETHANE SCAFFOLDS; GENE-EXPRESSION; POTENTIAL ROLE; STROMAL CELLS; TISSUE; DIFFERENTIATION; Mesenchymal stem cells; endothelial progenitor cells; bone; neovascularisation; 3D; co-cultures; autologous; platelet lysates |
| Subjects: | 600 Technology > 610 Medical sciences Medicine |
| Divisions: | Medicine > Lehrstuhl für Unfallchirurgie |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 06 Apr 2020 12:51 |
| Last Modified: | 06 Apr 2020 12:51 |
| URI: | https://pred.uni-regensburg.de/id/eprint/16394 |
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