Vaishampayan, Parag and Moissl-Eichinger, Christine and Puka, Ruediger and Schumann, Peter and Sproeer, Cathrin and Augustus, Angela and Roberts, Anne Hayden and Namba, Greg and Cisneros, Jessica and Salmassi, Tina and Venkateswaran, Kasthuri (2013) Description of Tersicoccus phoenicis gen. nov., sp nov isolated from spacecraft assembly clean room environments. INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 63. pp. 2463-2471. ISSN 1466-5026, 1466-5034
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Two strains of aerobic, non-motile, Gram-reaction-positive cocci were independently isolated from geographically distinct spacecraft assembly clean room facilities (Kennedy Space Center, Florida, USA and Centre Spatial Guyanais, Kourou, French Guiana). A polyphasic study was carried out to delineate the taxonomic identity of these two isolates (1P05MA(T) and KO_PS43). The 16S rRNA gene sequences exhibited a high similarity when compared to each other (100%) and lower than 96.7% relatedness with Arthrobacter crystallopoietes ATCC 15481(T), Arthrobacter luteolus ATCC BAA-272(T), Arthrobacter tumbae DSM 16406(T) and Arthrobacter subterraneus DSM 17585(T). In contrast with previously described Arthrobacter species, the novel isolates maintained their coccidal morphology throughout their growth and did not exhibit the rod coccus life cycle typically observed in nearly all Arthrobacter species, except A. agilis. The distinct taxonomic identity of the novel isolates was confirmed based on their unique cell-wall peptidoglycan type (A.11.20; Lys-Ser-Ala(2)) and polar lipid profile (presence of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, an unknown phospholipid and two unknown glycolipids). The G+C content of the genomic DNA was 70.6 mol%. The novel strains revealed MK-9(H-2) and MK-8(H-2) as dominant menaquinones and exhibited fatty acid profiles consisting of major amounts of anteiso-C-15:0 and anteiso-(17:0) and moderate amounts of iso-C-15:0 discriminating them again from closely related Arthrobacter species. Based on these observations, the authors propose that strains 1P05MA(T) and KO_PS43 be assigned into a separate genus Tersicoccus gen. nov. For this new taxon, comprising strains 1P05MA(T) and KO_PS43, we propose the name Tersicoccus phoenicis gen. nov., sp. nov. (the type species of Tersicoccus), represented by the type strain Tersicoccus phoenicis 1P05MA(T) (=NRRL B-59547(T)=DSM 30849(T)).
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | POLAR LIPID-COMPOSITION; COMB. NOV; COHN 1872; ARTHROBACTER; CLASSIFICATION; MICROORGANISMS; MICROCOCCUS; BACTERIA; SYSTEM; WATER; |
| Subjects: | 500 Science > 570 Life sciences |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 06 Apr 2020 13:11 |
| Last Modified: | 06 Apr 2020 13:11 |
| URI: | https://pred.uni-regensburg.de/id/eprint/16402 |
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