Hauptmann, Judith and Dueck, Anne and Harlander, Simone and Pfaff, Janina and Merkl, Rainer and Meister, Gunter (2013) Turning catalytically inactive human Argonaute proteins into active slicer enzymes. NATURE STRUCTURAL & MOLECULAR BIOLOGY, 20 (7). 814-+. ISSN 1545-9993,
Full text not available from this repository. (Request a copy)Abstract
Argonaute proteins interact with small RNAs that guide them to complementary target RNAs, thus leading to inhibition of gene expression. Some but not all Argonaute proteins are endonucleases and can cleave the complementary target RNA. Here, we have mutated inactive human Ago1 and Ago3 and generated catalytic Argonaute proteins. We find that two short sequence elements at the N terminus are important for activity. In addition, PIWI-domain mutations in Ago1 may misarrange the catalytic center. Our work helps in understanding of the structural requirements that make an Argonaute protein an active endonucleolytic enzyme.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | PASSENGER-STRAND; MESSENGER-RNA; GUIDE RNA; CLEAVAGE; BIOGENESIS; SIRNA; PLATFORM; PATHWAY; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biophysik und physikalische Biochemie > Prof. Dr. Rainer Merkl Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie I > Prof. Dr. Gunter Meister |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 07 Apr 2020 09:06 |
| Last Modified: | 07 Apr 2020 09:06 |
| URI: | https://pred.uni-regensburg.de/id/eprint/16458 |
Actions (login required)
 |
View Item |
