Comparison of the differentiation potential of neural crest derived progenitor cells from apical papilla (dNC-PCs) and stem cells from exfoliated deciduous teeth (SHED) into mineralising cells

Gosau, Martin and Goetz, Werner and Felthaus, Oliver and Ettl, Tobias and Jaeger, Andreas and Morsczeck, Christian (2013) Comparison of the differentiation potential of neural crest derived progenitor cells from apical papilla (dNC-PCs) and stem cells from exfoliated deciduous teeth (SHED) into mineralising cells. ARCHIVES OF ORAL BIOLOGY, 58 (6). pp. 699-706. ISSN 0003-9969,

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Abstract

Objective: Recently, cells from the apical papilla of retained human third molars (dental neural crest-derived progenitor cells, dNC-PCs) have been isolated and characterised as multipotent progenitor cells. Nonetheless, molecular processes during differentiation into mineralising cells are still unknown. This study evaluated the osteogenic/odontogenic differentiation of dNC-PCs under in vitro conditions and compared these cells with already known odontoblast precursor cells (dental stem cells from exfoliated human deciduous teeth, SHED). Methods: The differentiation of dNC-PCs and SHED under in vitro conditions was verified by Alizarin red staining (mineralisation), alkaline phosphatase activity and the expression of osteogenic/odontogenic markers (RT-PCRs). The genome wide expression-profiles were investigated with Affymetrix DNA-microarrays and the cell migration with a gel spot cell migration assay. Results: In our study dNC-PCs differentiated like SHED in mineralising cells. The expression of odontoblast markers suggested that dNC-PCs and SHED differentiated into different types of odontoblasts. This supposition was supported by genome wide gene expression profiles of dNC-PCs and SHED after cell differentiation. Typical biological processes of undifferentiated cells, for example "mitosis", were regulated in dNC-PCs. In SHED biological processes like "response to wounding" or "cell migration" were regulated, which are associated with replacement odontoblasts and their precursors. Moreover, a gel-spot assay revealed that SHED migrated faster than dNC-PCs. Conclusion: Our results suggest that dNC-PCs are precursors for primary odontoblasts, whereas SHED differentiate into replacement odontoblasts. These different odontogenic differentiation potentials of dNC-PCs and SHED have to be considered for cellular therapies and tissue engineering approaches in the future. (C) 2012 Elsevier Ltd. All rights reserved.

Item Type: Article
Uncontrolled Keywords: EXPRESSION; ODONTOBLASTS; PROTEINS; BIOLOGY; GROWTH; ROLES; Dental stem cells; Odontogenic differentiation; Osteogenic differentiation; Dental neural crest-derived progenitor cells; SHED; Microarray analysis
Subjects: 600 Technology > 610 Medical sciences Medicine
Divisions: Medicine > Lehrstuhl für Mund-, Kiefer- und Gesichtschirurgie
Depositing User: Dr. Gernot Deinzer
Date Deposited: 08 Apr 2020 13:53
Last Modified: 08 Apr 2020 13:53
URI: https://pred.uni-regensburg.de/id/eprint/16582

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