Hummel, Irena Brunskole and Reinartz, Michael T. and Kaelble, Solveig and Burhenne, Heike and Schwede, Frank and Buschauer, Armin and Seifert, Roland (2013) Dissociations in the Effects of beta(2)-Adrenergic Receptor Agonists on cAMP Formation and Superoxide Production in Human Neutrophils: Support for the Concept of Functional Selectivity. PLOS ONE, 8 (5): e64556. ISSN 1932-6203,
Full text not available from this repository. (Request a copy)Abstract
In neutrophils, activation of the beta(2)-adrenergic receptor (beta(2)AR), a G(s)-coupled receptor, inhibits inflammatory responses, which could be therapeutically exploited. The aim of this study was to evaluate the effects of various beta(2)AR ligands on adenosine-3',5'-cyclic monophosphate (cAMP) accumulation and N-formyl-L-methionyl-L-leucyl-L-phenylalanine (fMLP)induced superoxide anion (O-2(center dot-)) production in human neutrophils and to probe the concept of ligand-specific receptor conformations (also referred to as functional selectivity or biased signaling) in a native cell system. This is an important question because so far, evidence for functional selectivity has been predominantly obtained with recombinant systems, due to the inherent difficulties to genetically manipulate human native cells. cAMP concentration was determined by HPLC/tandem mass spectrometry, and O-2(center dot-) formation was assessed by superoxide dismutase-inhibitable reduction of ferricytochrome c. beta(2)AR agonists were generally more potent in inhibiting fMLP-induced O-2(center dot-) production than in stimulating cAMP accumulation. (-)-Ephedrine and dichloroisoproterenol were devoid of any agonistic activity in the cAMP assay, but partially inhibited fMLP-induced O-2(center dot-) production. Moreover, (-)-adrenaline was equi-efficacious in both assays whereas the efficacy of salbutamol was more than two-fold higher in the O-2(center dot-) assay. Functional selectivity was visualized by deviations of ligand potencies and efficacies from linear correlations for various parameters. We obtained no evidence for involvement of protein kinase A in the inhibition of fMLP-induced O-2(center dot-) production after beta(2)AR-stimulation although cAMP-increasing substances inhibited O-2(center dot-) production. Taken together, our data corroborate the concept of ligand-specific receptor conformations with unique signaling capabilities in native human cells and suggest that the beta(2)AR inhibits O-2(center dot-) production in a cAMP-independent manner.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | DEPENDENT PROTEIN-KINASE; BETA(2) ADRENERGIC-RECEPTOR; FORMING NADPH OXIDASE; CYCLIC-AMP; SIGNAL-TRANSDUCTION; RESPIRATORY BURST; COUPLED RECEPTORS; ADENYLATE-CYCLASE; OXIDATIVE BURST; ACTIVATION; |
| Subjects: | 600 Technology > 615 Pharmacy |
| Divisions: | Chemistry and Pharmacy > Institute of Pharmacy > Pharmaceutical/Medicinal Chemistry II (Prof. Buschauer) |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 09 Apr 2020 05:26 |
| Last Modified: | 09 Apr 2020 05:26 |
| URI: | https://pred.uni-regensburg.de/id/eprint/16646 |
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