Zillner, Karina and Filarsky, Michael and Rachow, Katrin and Weinberger, Michael and Laengst, Gernot and Nemeth, Attila (2013) Large-scale organization of ribosomal DNA chromatin is regulated by Tip5. NUCLEIC ACIDS RESEARCH, 41 (10). pp. 5251-5262. ISSN 0305-1048,
Full text not available from this repository. (Request a copy)Abstract
The DNase I accessibility and chromatin organization of genes within the nucleus do correlate to their transcriptional activity. Here, we show that both serum starvation and overexpression of Tip5, a key regulator of ribosomal RNA gene (rDNA) repression, dictate DNase I accessibility, facilitate the association of rDNA with the nuclear matrix and thus regulate large-scale rDNA chromatin organization. Tip5 contains four AT-hooks and a TAM (Tip5/ARBP/MBD) domain, which were proposed to bind matrix-attachment regions (MARs) of the genome. Remarkably, the TAM domain of Tip5 functions as nucleolar localization and nuclear matrix targeting module, whereas AT-hooks do not mediate association with the nuclear matrix, but they are required for nucleolar targeting. These findings suggest a dual role for Tip5's AT-hooks and TAM domain, targeting the nucleolus and anchoring to the nuclear matrix, and suggest a function for Tip5 in the regulation of higher-order rDNA chromatin structure.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | REMODELING COMPLEX NORC; RNA-POLYMERASE-I; NUCLEAR-MATRIX; BINDING DOMAIN; NUCLEOLAR MATRIX; ACTIVE CHROMATIN; GENES; TRANSCRIPTION; SEQUENCES; GENOME; |
| Subjects: | 500 Science > 570 Life sciences |
| Divisions: | Biology, Preclinical Medicine > Institut für Biochemie, Genetik und Mikrobiologie > Lehrstuhl für Biochemie III > Prof. Dr. Gernot Längst |
| Depositing User: | Dr. Gernot Deinzer |
| Date Deposited: | 09 Apr 2020 07:28 |
| Last Modified: | 09 Apr 2020 07:28 |
| URI: | https://pred.uni-regensburg.de/id/eprint/16692 |
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